内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠炎性因子表达的影响

Effects of endothelial progenitor cell transplantation on the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning

目的分析内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠模型炎症因子表达的影响,为内皮祖细胞移植在临床中的应用提供参考。方法选取60只雄性SD大鼠作为实验动物,采取随机数字表法将大鼠分为对照组、百草枯组(模型组)和内皮祖细胞移植组(观察组),各20只,比较3组大鼠的肺组织损伤情况及血清炎性因子等指标的表达水平。结果3组大鼠的肺组织损伤评分和湿干重比比较差异有统计学意义(F=12.336、10.571,均P<0.05),模型组大鼠的肺组织损伤评分和湿干重比均高于观察组和对照组(均P<0.05),但观察组和对照组比较差异无统计学意义(均P>0.05);3组大鼠肺血清炎性因子表达水平比较差异有统计学意义(F=23.305、26.271、18.814、20.056,均P<0.05),观察组和模型组大鼠的血清炎性因子表达水平均高于对照组(均P<0.05),但观察组的血清炎性因子表达水平低于模型组(均P<0.05);3组大鼠的氧化应激指标比较差异有统计学意义(F=8.326、14.505,均P<0.05),模型组大鼠的丙二醛(MDA)明显高于观察组和对照组(均P<0.05),且超氧化物歧化酶(SOD)明显低于观察组和对照组(均P<0.05),但观察组和对照组氧化应激指标比较差异无统计学意义(均P>0.05)。结论内皮祖细胞移植可以降低百草枯中毒所致急性肺损伤大鼠模型炎症因子表达,具有较好的疗效。

Objective To analyse the effect of endothelial progenitor cell transplantation on the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning and to provide references for the clinical application of endothelial progenitor cell transplantation.Methods Sixty male SD rats were selected as experimental animals,and the rats were divided into the control group,paraquat group(model group)and endothelial progenitor cell transplantation group(observation group)by a random number table method.The tissue damage and expression level of serum inflammatory factors and other indicators were also compared.Results The lung tissue damage score and wet-dry weight ratio of the three groups of rats were significantly different(F=12.336,10.571,all P<0.05).The lung tissue damage score and wet-dry weight ratio of the model group were higher than those of the observation and control groups(all P<0.05),but no significant difference was found between the observation and control groups(all P>0.05).The lung serum inflammatory factor expression levels of the three groups of rats were significantly different(F=23.305,26.271,18.814,20.056,all P<0.05),and the expression levels of the serum inflammatory factors in the observation and model groups were higher than those in the control group(all P<0.05),but the expression level of the serum inflammatory factors in the observation group was lower than that in the model group(all P<0.05).The oxidative stress indicators of the three groups of rats were significantly different(F=8.326,14.505,all P<0.05).The MDA of the model group was significantly higher than that of the observation and control groups(all P<0.05),and SOD of the model group was significantly lower than that of the observation and control groups(all P<0.05),but the observation and control groups had no significant difference in oxidative stress indicators(all P>0.05).Conclusion Endothelial progenitor cell transplantation can reduce the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning,which has a definite effect.