过度煎炸油诱发大鼠肝脏损伤及其机理研究

Deep-Frying Oil Induced Liver Injury in Rats and Its Mechanism Studies

通过采用定量灌胃的方式研究过度煎炸油(DFEO)的摄入对大鼠肝脏损伤的影响及其机理。研究发现:干预6周后,DFEO组肝脏指数显著下降(P<0.05),血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)水平显著升高(P<0.01)。肝组织切片观察发现,DFEO组出现大量脂肪空泡,肝窦扩张,肝细胞界限不清,局部腺体出现炎性细胞浸润。RT-PCR结果表明:连续食用过度煎炸油会导致大鼠肝脏组织Gsta2、Gsta3、Gclc、Gclm基因显著下调(P<0.01)。因此,摄入过度煎炸油会破坏肝细胞正常的组织形态,长期摄入会破坏正常的基因表达,影响正常的生命活动。

The effects of deep-frying edible oil(DFEO)on liver injury in rats and its mechanism were studied using oralgavage of DFEO with given doses.After 6 weeks of intervention,liver index in the DFEO group decreased significantly(P<0.05),while the serum levels of aspartate transaminase(ALT)and aspartate aminotrans ferase(AST)significantly increased(P<0.01).Meanwhile,observation of tissue sections of liver showed that a large number of fat vacuoles and liver sinusoidal expansion in the DFEO group.However,liver cell boundaries were not clear and inflammatory cell infiltration appearedin local glands.The quantitative real-time PCR results showed that 6 weeks continuous consumption of DFEO would cause significant down-regulation of Gsta2、Gsta3、Gclc、Gclm in rat liver tissues(P<0.01).DFEO will cause serious damage to the liver tissue and destroyliver cell morphology,and its long-term intake will damage the normal gene expression leading tonormal life activities affection.