摘要
为了解我国鸡滑液囊支原体(Mycoplasma synoviae,MS)感染的最新流行情况,本研究于2016―2019年从江苏、安徽、山东、河南、河北、宁夏、黑龙江、湖北等8个省份疑似发生MS感染的鸡场采集样品,进行MS菌株的分离鉴定,结果共获得48株MS分离株。对这些分离株的vlhA基因片段测序和分析显示,其中46株均属于K基因型,另外2株分别属于A基因型和E基因型。研究结果表明,当前国内流行的MS优势基因型是K基因型,与其他国家和地区流行的基因型存在显著性差异。本研究结果为制定适合我国鸡群MS控制与净化的策略提供了必要的流行病学依据。
In order to understand the latest prevalence of Mycoplasma synoviae(MS)infection in China,samples were collected from chicken farms suspected of infecting MS in 8 provinces including Jiangsu,Anhui,Shandong,Henan,Hebei,Ningxia,Heilongjiang and Hubei during 2016 to 2019 for the isolation and identification of MS strains.A total of 48 MS isolates were obtained.Sequencing and phylogenetic analysis of the vlhA gene of these isolates showed that 46 of them belonged to the K genotype,and the other 2 belonged to the A genotype and E genotype,respectively.These results showed that the prevailing dominant genotype in China was K genotype,which was significantly different from the prevailing genotype in other countries and regions.The results of this study provide the necessary epidemiological basis for formulating strategies suitable for the control and eradication of MS in chickens flocks in China.
作者
谢迪
马爽
楚电峰
孙通行
陈梦瑶
何吉鑫
吴艳涛
杜元钊
范根成
张小荣
XIE Di;MA Shuang;CHU Dianfeng;SUN Tongxing;CHEN Mengyao;HE Jixin;WU Yantao;DU Yuanzhao;FAN Gencheng;ZHANG Xiaorong(Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China;State Key Laboratory of Genetically Engineered Veterinary Vaccines,Qingdao Yebio Biological Engineering Co.,Ltd Qingdao,Shandong 266114,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第1期81-85,90,共6页
Chinese Journal of Veterinary Science
基金
动物基因工程疫苗国家重点实验室开放课题资助项目(AGVSKL-ZD-202001)
国家蛋鸡产业技术体系专项资助项目(CARS-40)
扬州大学高端人才支持计划资助项目(2016年)
江苏高校优势学科建设工程资助项目(2018年)。
关键词
滑液囊支原体
vlhA基因
K基因型
A基因型
E基因型
Mycoplasma synoviae
variable lipoprotein haemagglutinin gene(vlhA)
K genotype
A genotype
E genotype