超高效液相色谱法测定土壤中微量阿特拉津

Determination of Atrazine in Soil by Ultra High Performance Liquid Chromatography

采用超高效液相色谱仪,建立了土壤中微量阿特拉津的快速检测方法。研究结果表明:采用反相C18色谱柱,以甲醇/水(70∶30,v/v)为流动相,流速为0.2 ml/min,柱温为30℃,检测波长为220 nm条件下,在12.5~1000μg/L质量浓度范围内线性关系良好(r=0.9999),检出限0.18×10^(-3) mg/kg,加标回收率为69.0%~94.8%,相对标准偏差为2.5%~8.0%,该方法具有灵敏度高、重复性好、简便、快速、干扰小、精密度高的特点,可用于土壤中阿特拉津的快速检测。

A rapid method for the determination of trace atrazine in soil was established by high performance liquid chromatography.The optimized conditions were as follows:use C18 column,methanol-water(70∶30,v/v)as the mobile phase,flow rate is 0.2 ml/min,column temperature is 30℃,and detection wavelength is 220 nm.Under the optimized conditions,the calibration curve of atrazine was linear(r=0.9999)in the range of 12.5~1000μg/L with a detection limit of 0.18×10^(-3) mg/kg.The average spiked recoveries were between 69.0%to 94.8%with RSDs of 2.5%to 8.0%.The results indicated that the proposed method was easy to operate with little interference was well as high accuracy and stabilization,and was suitable for the determination of atrazine in soil.