哮喘-慢阻肺重叠综合征患者肠道菌群与血清炎症介质的相关性

Correlation between intestinal flora and serum inflammatory mediators in patients with asthma-COPD overlap syndrome

目的探讨哮喘-慢阻肺重叠综合征(ACOS)患者肠道菌群与血清炎症介质的相关性。方法选取ACOS患者176例为ACOS组,选取同期体检健康者120例为对照组。比较两组肠道菌群中双歧杆菌、乳酸杆菌、肠杆菌、肠球菌水平,比较血清肿瘤坏死因子-α(TNF-α)、白细胞介素-17(IL-17)、白细胞介素-8(IL-8)水平。通过Spearman相关性分析ACOS患者肠道菌群与血清炎症介质的关系。结果ACOS组肠道菌群中双歧杆菌、乳酸杆菌少于对照组(P<0.05),肠杆菌、肠球菌多于对照组(P<0.05);ACOS组患者血清TNF-α、IL-17、IL-8水平均显著高于对照组健康体检者(P<0.05);ACOS患者乳酸杆菌与TNF-α、IL-17、IL-8水平均呈负相关(r=-0.358、-0.366、-0.361,P<0.05),双歧杆菌与TNF-α、IL-17、IL-8水平均呈负相关(r=-0.479、-0.478、-0.500,P<0.05),肠杆菌与TNF-α、IL-17、IL-8水平均呈正相关(r=0.735、0.708、0.725,P<0.05),肠球菌与TNF-α、IL-17、IL-8水平均呈正相关(r=0.728、0.717、0.702,P<0.05)。结论ACOS患者炎症介质TNF-α、IL-17、IL-8水平明显升高,肠道菌群失衡,且肠道菌群平衡与炎症介质水平存在显著相关性。

Aim To investigate the correlation between intestinal flora and serum inflammatory mediators in patients with asthma-COPD overlap syndrome(ACOS).Methods 176 ACOS patients(ACOS group)and 120 healthy individuals(control group)in our hospital from December 2018 to June 2020 were enrolled.The levels of Bifidobacterium,Lactobacillus,Enterobacterium,Enterococcus,and serum levels of interleukin-8(IL-8),interleukin-17(IL-17)and tumor necrosis factor-α(TNF-α)were compared between groups.Spearman correlation analysis was used to analyze the relationship between intestinal flora and serum inflammatory mediators in ACOS patients.Results The number of Lactobacillus and Bifidobacterium in ACOS group was significantly less than that in control group(P<0.05),and the number of Enterobacterium and Enterococcus in the ACOS group was significantly higher than that in the control group(P<0.05).Serum levels of IL-8,IL-17 and TNF-αin ACOS group were significantly higher than those in control group(P<0.05).Lactobacillus in ACOS patients was negatively correlated with IL-8,IL-17,and TNF-αlevels(r=-0.358,-0.366,-0.361,P<0.05),and Bifidobacterium was negatively correlated with IL-8,IL-17,TNF-αlevels(r=-0.479,-0.478,-0.500,P<0.05),Enterobacterium was positively correlated with IL-8,IL-17,and TNF-αlevels(r=0.735,0.708,0.725,P<0.05),Enterococcus was positively correlated with IL-8,IL-17,and TNF-αlevels(r=0.728,0.717,0.702,P<0.05).Conclusion The elevated levels of inflammatory mediators IL-8,IL-17 and TNF-αin ACOS patients are associated with imbalance of intestinal flora.