侧柏叶对家兔心肌缺血-再灌注损伤的保护效应及其胆碱能抗炎作用

Study on the protective action of cacumen biotae on rabbits’myocardial ischemia reperfusion injury and its cholinergic anti-inflammatory function

目的探索侧柏叶(Cacumen Biotae,CB)对家兔心肌缺血-再灌注损伤(myocardial ischemia reperfusion injury,MIRI)的保护效应及胆碱能抗炎通路在此过程中的作用。方法将家兔随机分为假手术组、模型对照组、侧柏叶乙酸乙酯提取物组以及侧柏叶迷走神经切断组,每组8只,以心率、收缩压、血清肌酸激酶(creatine kinase,CK)、乳酸脱氢酶(lactic dehydrogenase,LDH)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、乙酰胆碱(acetyl choline,ACH)以及心肌组织总一氧化氮合成酶(nitric oxide synthetase,NOS)为检测指标,探讨CB对家兔MIRI的保护效应以及胆碱能抗炎通路在此过程中的作用。结果模型对照组家兔心率明显减慢、收缩压明显降低,血清心肌酶CK、LDH、血清TNF-α、IL-6和Ach水平明显升高(P<0.01),心肌组织NOS含量明显降低(P<0.01)。与模型对照组相比,侧柏叶乙酸乙酯提取物组能明显减小家兔MIRI心率和收缩压的降低率(P<0.01),显著减弱血清心肌酶CK和LDH的升高(P<0.01),明显降低血清TNF-α和IL-6水平(P<0.01),增加血清Ach及心肌组织NOS含量(P<0.01),而迷走神经切断后,侧柏叶的以上作用明显减弱(P<0.01)。结论侧柏叶对家兔MIRI具有保护作用,此作用与胆碱能抗炎通路有关。

Objective To study the protective action of Cacumen Biotae(CB)on rabbits’myocardial ischemia reperfusion injury and the cholinergic anti-inflammatory pathway’s function during this process.Methods Rabbits were divided into sham operation group,model control group,ethyl acetate extract group and CB plus vagus nerve cut group.Each group has 8 rabbits.Heart rate,blood pressure,blood levels of creatine kinase(CK),lactic dehydrogenase(LDH),tumor necrosis factor-α(TNF-α),interleukin(IL-6),acetyl choline(ACH)and the content of nitric oxide synthetase(NOS)in cardiac muscle were detected.According to these indexes,the protective action of CB on myocardial ischemia reperfusion injury and the cholinergic anti-inflammatory pathway’s function during this process were evaluated.Results For the rabbits in model control group,the blood pressure and heart rate reduced,The blood level of CK,LDH,TNF-α,IL-6 and ACH rose,the content of NOS in cardiac muscle decreased(P<0.01).For the rabbits in ethyl acetate extract group,the reduction ratio of heart rate and blood pressure after myocardial ischemia reperfusion injury minished obviously(P<0.01),the rise of blood level of CK and LDH weakened evidently(P<0.01),the blood level of TNF-α,IL-6 reduced and the blood level of ACH and the content of NOS in cardiac muscle increased markedly(P<0.01).After cutting the vagus nerve,these above functions of ethyl acetate extract of CB were weakened remarkablely(P<0.01).Conclusion CB has protective function on rabbits’myocardial ischemia reperfusion injury,and this function is closely related to the cholinergic anti-inflammatory pathway.