非小细胞肺癌患者外周血T淋巴细胞亚群与NK细胞活化性受体的表达及临床意义

Expression and clinical significance of lymphocyte subsets and NK cell activating receptors in peripheral blood of patients with non-small cell lung cancer

目的探讨非小细胞肺癌(NSCLC)患者外周血中淋巴细胞亚群比率分布、自然杀伤(NK)细胞表面活化性受体和调节性T细胞(Treg)的表达水平及临床意义。方法选取42例NSCLC患者,根据肿瘤分期分为Ⅰ期+Ⅱ期组和Ⅲ期+Ⅳ期组;根据分化程度分为高分化组、中分化组和低分化组。同时设良性病变组和健康对照组。采用流式细胞术检测外周血淋巴细胞亚群、NK细胞细胞毒性性受体3(NCR3/NKp30)和NCR1/NKp46和Treg表达水平。结果与健康对照组相比,Ⅲ期+Ⅳ期组患者CD3^(+)T细胞比例显著增加;Ⅲ期+Ⅳ期组和高分化组患者CD3^(+)CD4^(+)T细胞比例显著增加;各分期组和低分化组CD4^(+)T细胞中Treg比例增加;Ⅰ期+Ⅱ期组和中分化组NK细胞比例显著增加;各分期组和中、低分化组NK细胞NKp30和NKp46表达明显降低。结论NSCLC患者外周血T细胞、Treg、NK细胞比例增加,但NK细胞表面其活化性受体水平降低。

Objective To explore the expression and clinical significance of lymphocyte subsets distribution,NK cell activating receptors and regulatory T cells(Tregs)in the peripheral blood of patients with non-small cell lung cancer(NSCLC).Methods A total of 42 NSCLC patients were selected and divided into stageⅠ+Ⅱgroup and stageⅢ+Ⅳgroup according to tumor stage,or well-differentiated group,moderately-differentiated group and poorly-differentiated group according to the degree of differentiation.Noncancerous lesion group and healthy control group were also set up.Flow cytometry was used to detect the expression levels of lymphocyte subsets,NK cell cytotoxic receptor 3(NCR3/NKp30),NCR1/NKp46 and Tregs in the peripheral blood.Results Compared with the healthy control group,the proportion of CD3+T cells significantly increased in the stageⅢ+Ⅳgroup,the proportion of CD3^(+)CD4^(+)T cells also significantly increased in the stageⅢ+Ⅳgroup and the well-differentiated group,and the proportion of Treg in CD4^(+)T cells went up in all stage groups and poorly-differentiated group.The proportion of NK cells was significantly up-regulated in the stageⅠ+Ⅱgroup and the moderately-differentiated group.The expression of NKp30 and NKp46 on the surface of NK cells was significantly reduced in all stage groups,moderately-and poorly-differentiated groups.Conclusion The proportions of T cells,Tregs and NK cells increase,but the levels of activating receptors on NK cells decrease in the peripheral blood of patients with NSCLC.