MIP-3α、CCR6表达预测小细胞肺癌患者胸腔镜肺癌根治术后复发转移的价值

The Value of MIP-3 and CCR6 Expression in Predicting Recurrence and Metastasis of Small Cell Lung Cancer after Thoracoscopic Radical Resection

目的探讨小细胞肺癌(small cell lung cancer,SCLC)患者巨噬细胞炎性蛋白-3α(MIP-3α)、趋化因子C-C-基元受体6(CCR6)表达在预测胸腔镜肺癌根治术后复发转移中的临床价值。方法选取SCLC患者160例,术后随访2 a,根据术后是否发生复发转移分为转移组(n=70)和非转移组(n=90)。采用免疫化学染色检测MIP-3α、CCR6的表达;采用实时荧光定量PCR检测MIP-3α、CCR6 mRNA的表达,采用蛋白质印迹法检测MIP-3α、CCR6蛋白的表达。结果转移组MIP-3α、CCR6 mRNA分别为0.88±0.12、0.64±0.10,蛋白表达水平分别为3.92±0.13、2.87±0.35;非转移组MIP-3α、CCR6 mRNA分别为0.48±0.09、0.41±0.05,蛋白表达水平分别为1.00±0.00、1.01±0.00,转移组MIP-3α、CCR6 mRNA及蛋白表达水平高于非转移组,差异有统计学意义(P<0.05)。结论MIP-3α、CCR6在经胸腔镜肺癌根治术SCLC患者术后复发转移组织中高度表达,MIP-3α、CCR6可为SCLC的临床治疗提供参考。

Objective To explore the clinical value of MIP-3αand CCR6 expression in predicting the recurrence and metastasis of patients with small cell lung cancer(SCLC).Methods 160 patients with SCLC were selected.Follow-up for 2 years,the patients were divided into observation group(n=70)and control group(n=90)according to whether recurrence and metastasis.The expression of MIP-3αand CCR6 was detected by immunochemical staining,MIP-3αand CCR6 mRNA by real-time fluorescence quantitative PCR,MIP-3αand CCR6 protein by western blotting.Results In the observation group,MIP-3αand CCR6 mRNA were 0.88±0.12,0.64±0.10 and protein were 3.92±0.13 and 2.87±0.35,respectively;in the control group,MIP-3αand CCR6 mRNA were 0.48±0.09 and 0.41±0.05 and protein were 1.00±0.00 and 1.01±0.00.The expression of MIP-3αand CCR6 in the observation group was significantly higher than that in the control group,the difference between the two groups has statistical significance(P<0.05).Conclusion MIP-3 and CCR6 are highly expressed in recurrent and metastatic tissues of SCLC patients undergoing radical thoracoscopic surgery,and MIP-3αand CCR6 can provide references for the clinical treatment of SCLC.