应用溴甲酚紫建立贵州省辣椒炭疽病菌快速选药技术研究

Establishment of Technology of Rapid Fungicides Selection against Colletotrichum gloeosporioides in Guizhou Province Using Bromocresol Purple

为了建立辣椒炭疽病快速选药技术,对贵州省4个辣椒主产区100株辣椒炭疽病菌菌株进行敏感性测定,建立了贵州省辣椒炭疽病菌对甲基硫菌灵和咪鲜胺的敏感基线,并以敏感基线为基准,应用溴甲酚紫为指示剂,研制出辣椒炭疽病菌快速选药试剂盒。结果表明,贵州省辣椒炭疽病菌对甲基硫菌灵和咪鲜胺的敏感基线分别为(0.7641±0.1508)μg/mL和(0.0710±0.0133)μg/mL。尖孢炭疽菌在0.45μg/mL甲基硫菌灵的试剂盒上颜色显示为橙红色表明比较敏感,在1.80μg/mL甲基硫菌灵的试剂盒上显示黄色则表明已经产生抗性,在0.088μg/mL咪鲜胺的试剂盒上显示橙红色表明比较敏感,在0.350μg/mL咪鲜胺的试剂盒上显示黄色则表明已经产生抗性。

In order to establish the technology of rapid fungicides selection against Colletotrichum gloeosporioides,100 isolates of C.gloeosporioides were isolated and collected from Guizhou.The sensitivity baslines of C.gloeosporioides to thiophanate methyl and prochloraz were determined by mycelial growth rate method.Based on sensitivity basline of C.gloeosporioides to thiophanate methyl and prochloraz,the rapid fungicides selection kit against C.gloeosporioides was established using bromocresol purple.The results showed that the EC50 mean values of(0.7641±0.1508)μg/mL and(0.0710±0.0133)μg/mL were sensitivity baslines of C.gloeosporioides to thiophanate methyl,prochloraz in Guizhou region,respectively.When the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 0.45μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to thiophanate methyl was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of thiophanate methyl of 1.80μg/mL,the color of the kit was yellow,indicating that the strain was resistant.When the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.088μg/mL,the color of the kit was orange red,indicating that the strain of C.gloeosporioides to prochloraz was sensitive.But when the strain of C.gloeosporioides was placed in selection kit of prochloraz of 0.350μg/mL,the color of the kit was yellow,indicating that the strain was resistant.