硒对氧化应激诱导的胎盘滋养层细胞增殖与凋亡的影响

Effects of Selenium on Proliferation and Apoptosis of Placental Trophoblast Cells Induced by Oxidative Stress

目的:检测硒(NaSe)对CoCl2氧化应激诱导人胎盘滋养层细胞(JEG-3)增殖与凋亡的影响及其可能机制。方法:体外培养JEG-3细胞,在加入CoCl2(500μM)氧化应激诱导前先加入NaSe(100nM)预处理24小时,MTT实验检测硒对氧化应激JEG-3的增殖促进作用;利用细胞流式术(FCM)检测硒对氧化应激JEG-3细胞凋亡的影响;用Western blot检测硒影响氧化应激JEG-3细胞增殖与凋亡的可能分子生物学机制。结果:MTT提示硒能够增加氧化应激诱导的JEG-3细胞的增殖活性(P<0.05),降低氧化应激JEG-3细胞凋亡率(P<0.01),同时硒蛋白Gpx1表达上调(P<0.05),脂质过氧化物MDA表达下降(P<0.05)。结论:硒通过上调硒蛋白Gpx1表达,降低脂质过氧化物MDA表达,进而降低氧化应激JEG-3细胞凋亡率而发挥其促进增殖活性,提示硒的补充对子痫前期的预防和治疗具有重要的意义。

Objective: To examine the effect of selenium(NaSe) on the proliferation and apoptosis of human placental trophoblast cells(JEG-3) induced by CoCl2 oxidative stress. Methods: JEG-3 cells were cultured in vitro and pretreated with nase(100 nm) for 24 hours before CoCl2(500 μm) oxidative stress was added. MTT assay was used to detect the effect of selenium on the proliferation of JEG-3 under oxidative stress;Cell flow cytometry(FCM) was used to detect the effect of selenium on apoptosis of JEG-3 cells under oxidative stress;Western blot was used to detect the possible molecular biological mechanism of selenium on proliferation and apoptosis of JEG-3 cells under oxidative stress. Results: MTT suggested that selenium could increase the proliferation of JEG-3 cells induced by oxidative stress(P<0.05), and decrease the apoptosis rate of JEG-3 cells(P<0.01);at the same time, the expression of selenoprotein Gpx1 was up-regulated(P<0.05), and the expression of MDA was decreased(P<0.05). Conclusions: Selenium promotes the proliferation of JEG-3 cells by up regulating the expression of Gpx1 and decreasing the expression of MDA, which indicates that selenium supplementation is of great significance in the prevention and treatment of preeclampsia.