荧光光谱标准加入法快速检测雪碧中的苯甲酸钠

Rapid Determination of Sodium Benzoate in Sprite by Fluorescence Spectrum Standard Addition Method

为建立一种快速测定雪碧中苯甲酸钠的方法,探讨了雪碧体系中苯甲酸钠的荧光特性,建立了标准加入法检测雪碧中的苯甲酸钠的方法,并对该方法进行了评价。结果表明:荧光检测最佳的激发波长为265nm、发射波长为313nm;100mL检测体系中,雪碧添加量为10mL,盐酸浓度0.01mol/L、表面活性剂浓度0.05g/L,当加入0~20mL0.1g/L苯甲酸钠溶液时,荧光强度与苯甲酸钠的加入体积有良好的线性关系,工作曲线为y=39.8x+638.3,相关系数为0.9991,检出限为9.07×10^(-5)g/L,变异系数为1.1%。

To establish a method for direct determination of sodium benzoate in Sprite,the fluorescence properties of sodium benzoate in Sprite are studied.A standard addition method is developed for the determination of sodium benzoate in sprite,and the method is evaluated.The results show that the optimum excitation wavelength and emission wavelength are 265 nm and 313 nm respectively for fluorescence detection.In the 100 mL detection system,the addition of Sprite is 10 mL,the concentration of hydrochloric acid is 0.01 mol/L,and the concentration of surfactant is 0.05 g/L.When 0~20 mL 0.1 g/L sodium benzoate solution is added,there is a good linear relationship between fluorescence intensity and the adding volume of sodium benzoate.The working curve is y=39.8 x+638.3;the correlation coefficient is 0.9991;the detection limit is 9.07×10^(-5)g/L;and the coefficient of variation is 1.1%.