卵巢癌组织中miR-377的表达及其与临床病理参数的关系

Expression level of miR-377 in ovarian cancer tissues,and its relationship with clinicopathological parameters

目的:考察卵巢癌组织中miR-377的表达,分析miR-377表达与临床病理参数及预后的相关性。方法:收集卵巢癌组织及同期正常卵巢组织标本各50例,分别设为卵巢癌组及对照组,并收集病人临床病理参数资料。采用RT-qPCR技术检测2组样本的中miR-377的表达水平,分析miR-377与卵巢癌病人相关临床病理参数的关系,采用生存曲线图分析miR-377表达与预后的关系,并采用多因素COX回归分析卵巢癌预后的独立危险因素。结果:卵巢癌组miR-377表达低于对照组,差异有统计学意义(P<0.01)。卵巢癌组miR-377表达与肿瘤的TNM分期、临床分期、是否转移有关(P<0.05~P<0.01),与病人的年龄、卵巢癌类型等临床病理参数无关(P>0.05)。miR-377低表达者5年总生存率55.88%显著低于高表达者75.00%(P<0.01)。多因素COX回归分析显示,miR-377低表达与TNM分期是卵巢癌预后的独立危险因素(P<0.01)。结论:miR-377在卵巢癌病人组织中的表达显著降低,miR-377表达水平与卵巢癌病人的TNM分期、临床分期及病理分级有关,且miR-377低表达与TNM分期可视为卵巢癌预后的独立危险因素。

Objective:To investigate the expression level of miR-377 in ovarian cancer tissues,and analyze the correlation between miR-377 expression,and clinicopathological parameters and prognosis of patients.Methods:Fifty ovarian cancer tissue samples and 50 normal ovarian tissue samples were divided into the ovarian cancer group and control group,respectively.The clinical pathological parameters data were collected.The expression levels of miR-377 in two groups were detected using RT-qPCR,and the relationship between mir-377 expression and relevant clinical pathological parameters in ovarian cancer patients were analyzed.The relationship between mir-377 expression and prognosis was analyzed using survival curve,and the independent risk factors of ovarian cancer prognosis using multivariate COX regression method.Results:The expression level of miR-377 in ovarian cancer group was significantly lower than that in control group(P<0.01).The expression level of miR-377 in ovarian cancer group was correlated with the TNM stage,clinical stage and metastasis of tumor(P<0.05 to P<0.01),and not related to the age and ovarian cancer type of clinicopathological parameters(P>0.05).The 5-year survival rate of patient with low expression of miR-377(55.88%)was significantly lower than that in patients with high expression(75.00%)(P<0.01).The results of multivariate COX regression analysis showed that the low expression of miR-377 and TNM staging were the independent risk factors of ovarian cancer prognosis(P<0.01).Conclusions:The expression level of miR-377 in the ovarian cancer tissues decreases significantly.The expression levels of miR-377 is related to the TNM stage,clinical stage and pathological grade of ovarian cancer,and the low expression of miR-377 and TNM stage are the independent risk factors of the prognosis of patients with ovarian cancer.