罗非鱼原核表达基因研究进展

Advances in Prokaryotic Expression Gene of Tilapia

罗非鱼作为世界范围广泛养殖的经济鱼类,其营养价值特别高,是当前淡水养殖业的重要鱼类养殖品种之一。原核表达是利用基因克隆技术,把外源基因与特定表达载体结合,然后转入表达菌株,使基因在体外得以表达的一种技术。该技术可将特定基因翻译成蛋白质,为特定基因的蛋白水平研究提供基础材料。目前,随着罗非鱼养殖业的扩大,对罗非鱼的研究也日益深入,尤其是免疫、生长相关基因。原核表达在这些基因的蛋白水平研究中做出了巨大贡献。然而,原核表达技术涉及多种表达载体与表达宿主的匹配,不适宜的表达载体与表达宿主将导致表达失败。因此,综述了现有原核表达的罗非鱼基因,总结归纳了原核表达的基因类型,使用的载体和表达宿主以及表达蛋白的用途与局限性,以期为罗非鱼基因蛋白水平研究提供参考。

As a worldwide economic fish,tilapia contains rich nutrition and is one of the most important fish cultured in freshwater.Prokaryotic expression is a technology that combines foreign genes with the expression vectors by gene cloning technology and transfers the combined genes into expression strains in purpose of translating the goal gene into protein.Specific genes can be translated into protein by this technology,and thus provide basic materials for protein level research of specific genes.With the expansion of tilapia industry,researches on tilapia are increasing deeply,especially the genes related to immunity and growth.Prokaryotic expression has made great contribution to the study of these genes in protein level.However,prokaryotic expression needs the match of a suitable expression vector and host,and inappropriate expression vectors and hosts results in the failure of expression.This paper reviews the studied genes of tilapia by prokaryotic expression,summarizes the genes types,vectors and hosts for prokaryotic expression,as well as the uses and limitations of expressed protein,aiming to offer references for further researches of tilapia genes in protein level.