微小核糖核酸-7调控表皮细胞生长因子受体对肝癌细胞增殖和转移的影响

The effect of microRNA-7 regulating epidermal growth factor receptor on the proliferation and metastasis of hepatocellular carcinoma cells

目的探讨微小核糖核酸(miR)-7是否通过靶向调控表皮细胞生长因子受体(EGFR)对人肝癌细胞侵袭和增殖产生影响。方法传代培养HepG2人肝癌细胞,脂质体转染miR-7后使其表达上调或下调。荧光定量聚合酶链反应检测转染效率。蛋白印迹法检测miR-7上调或下调后肝癌细胞EGFR表达变化。溴化噻唑蓝四氮唑(MTT)法检测miR-7上调或下调后肝癌细胞增殖能力。划痕试验检测miR-7上调或下调后肝癌细胞迁移能力。结果脂质体转染HepG2人肝癌细胞可控制miR-7表达,miR-7表达与肝癌细胞EGFR表达呈负相关。miR-7表达上调时,肝癌细胞增殖和迁移变慢;反之,miR-7表达下调时,肝癌细胞增殖和迁移变快。结论 miR-7靶向调节EGFR表达,其表达与肝癌细胞增殖和迁移呈负相关。miR-7可能是未来肝癌治疗新策略的潜在靶点。

Objective To clarify the effect of microRNA-7(miR-7) on the invasion and proliferation of human hepatocellular carcinoma(HCC) cells through targeting regulation of epidermal growth factor receptor(EGFR). Methods Human HepG2 HCC cells were subcultured and miR-7 expression was up-regulated or down-regulated after miR-7 was transfected with liposome. The transfection efficiency of miR-7 expression was detected by real-time fluorescent quantitative polymerase chain reaction(PCR) method. The expressions of EGFR protein in HCC cells that were up-regulated or down-regulated by miR-7 were tested by Western Blot.MTT assay was used to test the proliferation ability of HCC cells that were up-regulated or down-regulated by miR-7. The migration ability of HCC cells that were up-regulated or down-regulated by miR-7 was checked by scratch test. Results The expression of miR-7 could be controlled by transfection of HepG2 HCC cells with liposomes,and the expression of miR-7 was negatively correlated with the expression of EGFR protein in HCC cells. When the expression of miR-7 was up-regulated,the proliferation and migration of HCC cells slowed down;on the contrary,when the expression of miR-7 was down-regulated,the proliferation and migration of HCC cells increased up. Conclusion MiR-7 can affect the expression of EGFR protein through targeting regulation,and the expression of EGFR bears a negative correlation with the proliferation and migration of HCC cells. Therefore,miR-7 may become a potential target for new therapeutic strategies for HCC in the future.(J Intervent Radiol,2021,30: 43-47)