硅酸铜纳米颗粒作为化学动力学治疗剂的研究

Study on copper silicate nanoparticles as chemokinetic therapeutic agents

目的将Cu2+掺杂于介孔二氧化硅纳米颗粒,制备出可促进肿瘤细胞内芬顿反应、具有化学动力学疗效的硅酸铜纳米平台,并验证其肿瘤杀伤能力和细胞生物安全性。方法利用水热法,通过Cu2+掺杂介孔二氧化硅,制备出硅酸铜纳米粒子,并通过透射电子显微镜、X射线衍射、氮气吸附脱附曲线等检测手段表征纳米粒子的形貌、晶体结构、孔隙率和比表面积等物理化学性质;通过CCK-8细胞实验验证未掺杂Cu2+的介孔二氧化硅的细胞生物相容性;利用细胞实验评价该硅酸铜纳米颗粒在癌细胞中所产生的细胞毒性羟基自由基的数目,以及进一步评估该纳米颗粒对癌细胞的治疗效果。结果透射电子显微镜图显示介孔二氧化硅与硅酸铜纳米颗粒呈现出球形形貌,具有较好的单分散性,水合平均粒径分别为85.9、204.4 nm。氮气吸脱附曲线分析结果显示介孔二氧化硅与硅酸铜纳米颗粒的比表面积分别为26.8、5.36 m2/g,其纳米颗粒的孔径分别为18.9、1.66 nm。CCK-8细胞实验结果显示介孔二氧化硅纳米颗粒具有优异的生物相容性,而硅酸铜纳米颗粒对癌细胞展现出较强的细胞毒性。结论成功研究制备出硅酸铜纳米颗粒,该颗粒实现很好的抗肿瘤性能,具有较好的化学动力学治疗前景。

Objective By doping Cu2+ with mesoporous silica, a copper silicate nano platform with chemical kinetic effect was prepared, which can catalyze hydrogen peroxide to produce strong oxidative hydroxyl radicals, realize the oxidative damage of cancer cells and lead to cancer cells death.Methods By hydrothermal method, mesoporous silica was etched by copper ion to prepare copper silicate nanoparticles.The morphology, crystal structure, porosity, specific surface area and other physical and chemical properties of the nanoparticles were characterized by transmission electron microscope, X-ray diffraction and nitrogen adsorption desorption curve.Secondly, the biocompatibility of mesoporous silica without copper ions was verified by cell experiments.At the same time, the number of cytotoxic hydroxyl radicals produced by the copper silicate nanoparticles in cancer cells was evaluated by cell experiments,and the therapeutic effect of the nanoparticles on cancer cells was further evaluated.Results TEM images showed that mesoporous silica and copper silicate nanoparticles showed spherical morphology with good monodispersity.The average hydration particle sizes were 85.9 nm and 204.4 nm,respectively.The results of nitrogen adsorption desorption curve showed that the specific surface area of mesoporous silica and copper silicate nanoparticles were 26.8 m2/g and 5.36 m2/g,respectively,and the pore size of the nanoparticles was 18.9 nm and 1.66 nm,respectively.Subsequently,through cell experiments,we knowed that mesoporous silica nanoparticles had excellent biocompatibility,while copper silicate nanoparticles showed a strong inhibition on cancer cells.Conclusion In this study,copper silicate nanoparticles were successfully prepared and achieved good antitumor performance,indicating that they have a good prospect of chemokinetic therapy.