基于网络药理学对枸杞子-五味子治疗注意缺陷多动障碍的机制探究

Mechanism Study on the Investigation of the Mechanism of Fructus Lycii-Fructus Schisandrae Chinensis in the Treatment of Attention-deficit/Hyperactivity Disorder Based on Network Pharmacology

目的:基于网络药理学探讨枸杞子-五味子药对治疗注意缺陷多动障碍的药效物质基础和作用机制。方法:检索ETCM数据库,筛选枸杞子-五味子活性成分及靶基因;检索Drugbank、Genecards、DisGeNET和TTD数据库,筛选ADHD相关靶基因;利用Cytoscape构建药对活性成分-靶点网络并进行拓扑结构分析;对疾病靶点与药对靶点绘制维恩图筛选关键靶点,利用David进行GO分析和KEGG分析。结果:筛选出枸杞子-五味子有36个活性成分,207个靶基因,活性成分-靶点拓扑结构分析得到Inosine和其他基因互相关联程度最高;筛选出ADHD相关靶基因1861个,维恩图提取关键靶点73个,GO分析得到11条生物过程,12条细胞组分表达过程,14个分子功能相关过程;KEGG分析显示21条信号通路,包括类固醇激素的合成、细胞色素P450代谢、GABA能突触及胆碱能突触调节、色氨酸代谢等。结论:枸杞子-五味子药对活性成分通过多成分、多靶点、多通路调控机体类固醇激素代谢、神经递质代谢、中枢神经系统调节、免疫调节对ADHD发挥治疗作用。

Objective:To explore the molecular mechanism of Fructus Lycii-Fructus Schisandrae Chinensis in the treatment of attention-deficit/hyperactivity disorder based on network pharmacology.Methods:ETCM was used to screen the active components and target gene of Fructus Lycii-Fructus Schisandrae Chinensis.Drugbank,Genecards,DisGeNET and TTD databases was searched to screen ADHD-related target genes;Cytoscape was used to construct a drug-to-active ingredient-target network and conduct topological structure analysis;Venn diagrams for disease targets and drug-to-targets were drawn to screen key targets.David was used for GO analysis and KEGG analysis.Results:A total of 36 active ingredients and 207 potential target genes related to Fructus Lycii-Fructus Schisandrae Chinensis were retrieved from ETCM.1861 ADHD-related target genes were screened,73 key targets were extracted from Venn diagrams.A total of 11 biological processes,12 cell component expression processes,and 14 molecular functions related processes were obtained by GO analysis;KEGG analysis showed 21 signal pathways,including the synthesis of steroid hormones,cytochrome P450 metabolism,GABAergic synapse and cholinergic synaptic regulation,tryptophan metabolism,etc.Conclusion:The active ingredients of Fructus Lycii-Fructus Schisandrae Chinensis active ingredients can regulate the body′s steroid hormone metabolism,neurotransmitter metabolism,central nervous system regulation,and immune regulation through multiple components,multiple targets,and multiple pathways to play a therapeutic effect on ADHD.