摇法对KOA兔滑膜MMPs分泌功能的作用研究

Study on the secretion function of MMPs in KOA rabbit synovial membrane by rocking manipulation

[目的]研究推拿摇法对膝骨性关节炎(KOA)兔膝关节滑膜炎症影响及对滑膜基质金属蛋白酶(MMPs)分泌功能的作用。[方法]将50只新西兰大耳白兔编号后随机分为5组,其中模型组、塞来昔布组、摇法组采用关节腔木瓜蛋白酶注射造模,假模型组关节腔等剂量生理盐水注射,正常组不做干预,同条件、同期饲养,取材后HE染色观察滑膜组织炎性浸润程度;酶联免疫吸附(ELISA)法检测各组滑膜液内基质金属蛋白酶1(MMP1)、基质金属蛋白酶13(MMP13)的表达。[结果]苏木精-伊红(HE)染色显示,模型组滑膜组织增厚,滑膜细胞形态异常,炎性细胞浸润,血管翳形成;摇法组滑膜轻度增厚,细胞形态基本正常,炎性浸润较模型组明显改善;塞来昔布组滑膜增厚4~5层,衬里细胞扁平,炎症浸润较模型组明显改善。ELISA法检测各组滑膜液中MMP1、MMP13的表达,假模型组与正常组MMP1、MMP13的表达经统计学比较,无统计学意义(P>0.05),模型组与正常组比较,差异具有统计学意义(P<0.05),塞来昔布组与模型组比较,差异具有统计学意义(P<0.05),摇法组与模型组比较,差异具有统计学意义(P<0.05),塞来昔布组与摇法组相比,无统计学意义(P>0.05)。[结论]摇法具有缓解滑膜炎症浸润,抑制MMP1、MMP13分泌的作用,摇法可以通过缓解滑膜炎性反应降低KOA软骨基质分解代谢,减轻KOA症状。

[Objective]To study the effect of rocking manipulation on synovial inflammation and secretion of synovial MMPs in KOA rabbits.[Methods]50 New Zealand big-eared white rabbits were randomly divided into 5 groups,Model group,Celecoxib group,Rocking manipulation group were using papain articular cavity injection mould,False model group joint cavity isodose physiological saline injection,all group were raised in the same conditions,at the same time,based on HE staining observing synovial tissue inflammatory invasion degree.The expressions of MMP1 and MMP13 in synovial fluid were detected by ELISA.[Results]HE staining showed that the synovial tissue of the model group was thickened,the morphology of synovial cells was abnormal,inflammatory cells invaded,and pannus formation was formed.The synovial membrane was slightly thickened,the cell morphology was basically normal,and the inflammatory invasion was significantly improved compared with the model group.The synovial membrane of the celecoxib group was thickened by 4~5 layers,the lining cells were flattened,and the inflammatory invasion was significantly improved compared with the model group.Constituting the ELISA method to detect the expression of MMP1 and MMP13 in synovial fluid,false model group and normal group,the expression of MMP1 and MMP13 via statistical comparison,no significant(P>0.05),compared with normal group,model group with significant difference(P<0.05),celecoxib group compared with model group,with significant difference(P<0.05),rocking manipulation group compared with model group,with significant difference(P<0.05),celecoxib group compared with the rocking manipulation group,no significant(P>0.05).[Conclusion]Rocking manipulation can alleviate synovial inflammation invasion,inhibit the secretion of MMP1 and MMP13,and reduce the catabolism of KOA cartilage matrix and KOA symptoms by alleviating synovial inflammation.