两种检验方法在乙肝病毒检验中的应用分析

Application Analysis of Two Test Methods in Hepatitis B Virus Test

目的比较化学发光法与酶联免疫吸附法在乙肝病毒检验中的应用效果。方法选取2019年4月至2020年3月诸城市妇幼保健院收治的64例乙型肝炎患者为研究对象。所有患者均取空腹静脉血,分别进行化学发光法与酶联免疫吸附法进行血清标志物的检验。比较两种检验方法乙型肝炎表面抗原(HBsAg)、乙型肝炎表面抗体(HBsAb)、乙型肝炎e抗原(HBeAg)、乙型肝炎e抗体(HBeAb)和乙型肝炎核心抗体(HBcAb)的阳性检出率、确诊率以及不同样本浓度下的检出率。结果化学发光法HBsAg、HBeAg和HBeAb阳性检出率均优于酶联免疫吸附法(P<0.05);而两种检测方法的HBsAb、HBcAb阳性检出率比较,差异无统计学意义(P>0.05);化学发光法确诊率92.19%高于酶联免疫吸附法的确诊率78.13%(P<0.05)。结论采用化学发光法进行血清标志物检验,阳性检出率高于酶联免疫吸附法。由于患者体内乙肝病毒活跃度不同,其血清标志物滴度不同,因此采用两种检验方法的结果均有误差,但化学发光法误差较小,更适合用于乙肝病毒的检验。

Objective To compare the application effect of chemiluminescence method and enzyme-linked immunosorbent assay in the detection of hepatitis B virus.Methods A total of 64 hepatitis B patients admitted to Zhucheng Maternal and Child Health Hospital from April 2019 to March 2020 were selected as the study subjects.Fasting venous blood was collected from all patients,and serum markers were tested by chemiluminescence and enzyme-linked immunosorbent assay.The positive detection rate and diagnostic rate of hepatitis B surface antigen(HBsAg),hepatitis B surface antibody(HBsAb),hepatitis B e antigen(HBeAg),hepatitis B e antibody(HBeAb)and hepatitis B core antibody(HBcAb)were compared between the two test methods,and the detection rate at different sample concentrations.Results The positive detection rates of HBsAg,HBeAg and HBeAb by chemiluminescence method were superior to those by enzyme-linked immunosorbent assay(P<0.05);while the positive detection rates of HBsAb and HBcAb by the two detection methods were not statistically significant(P>0.05);the positive diagnosis rate by chemiluminescence method was 92.19%higher than that by enzyme-linked immunosorbent assay 78.13%(P<0.05).Conclusion Chemiluminescence method was used to detect serum markers,and the positive detection rate was higher than that of ELISA.Due to the different activity of hepatitis B virus in patients and the different titers of serum markers,the results of the two test methods have errors,but the errors of chemiluminescence method are small,which is more suitable for the detection of hepatitis B virus.