壳聚糖絮凝蛹虫草菌丝体多糖工艺优化及其失活动力学分析

Optimization of chitosan flocculation of Cordyceps militaris polysaccharide and analysis of inactivation mechanics of chitosan

利用响应面法对蛹虫草诱变菌株CSYB-2菌丝体多糖的制备eé工艺进行优化,结果显示在壳聚糖用菌株CSYB-2菌丝体多糖浸提液的絮凝工艺基础上,通过构建壳聚糖失活动力学模型,探究絮凝剂(壳聚糖)在絮凝过程中的动力学规律和失活机理。结果表明壳聚糖的失活动力学符合一级反应的失活动力学方程,在考察溶液澄清率(絮凝率)在不同时间、温度下变化规律的基础上推算出失活速率常数、活化能等动力学函数值,为研究絮凝作用中絮凝剂失活的机理提供理论支持。

The response surface method was used to optimize the preparation process of Cordyceps militaris mutant strain CSYB-2 mycelium polysaccharides.The results showed that the polysaccharide retention rate was(82.05±0.21)%,and the RSD of the process stability test was 2.53%under the conditions of chitosan dosage of 1.4 mL/g,flocculation temperature of 55℃,and flocculation time of 70 min.Mycelium polysaccharide extract of chitosan flocculant mutated strain CSYB-2 was used to construct a chitosan inactivation mechanical model,and the dynamics of flocculant during flocculation and the mechanism of inactivation were investigated.The test indicated that the inactivation mechanics of chitosan conformed to the inertia mechanics equation of the first-order reaction.The inactivation rate constant and activation energy are calculated based on the changes in the solution clarification rate(flocculation rate)at different times.This study provides theoretical support for investigating the mechanism of flocculant deactivation in flocculation.