Survivin基因在肝癌细胞增殖、凋亡过程中的表达及意义

Expression of survivin gene in proliferation and apoptosis of hepatocellular carcinoma cells

目的探索Survivin基因在肝癌细胞增殖和凋亡过程中的表达及其临床意义。方法将肝癌细胞分为空白组(A组)、载体病毒组(B组)、shRNA-Survivin慢病毒组(C组),使用MTT法分别检测三组肝癌细胞转染后HepG2肝癌细胞增殖率、使用AV-PI凋亡试剂盒分别检测三组肝癌细胞凋亡抑制率、使用RT-qPCR检测Survivin基因相对表达量以及凋亡因子半胱氨酸天冬氨酸蛋白酶-3 (Caspase-3)的表达量。结果 A组、B组和C组肝癌细胞转染后HepG2肝癌细胞增殖率分别为(7.38±0.422)%、(8.99±0.382)%和(14.09±0.291)%,A组、B组的HepG2肝癌细胞增殖率明显低于C组,差异均具有统计学意义(P<0.05);A组、B组和C组肝癌细胞凋亡抑制率分别为(4.56±1.37)%、(3.05±1.08)%和(39.45±12.88)%,A组、B组肝癌细胞凋亡抑制率明显低于C组,差异均具有统计学意义(P<0.05);经荧光定量PCR检测,A组、B组和C组中Survivin基因相对表达量分别为0.938±0.049、0.979±0.068和0.488±0.085,A组、B组的Survivin基因相对表达量明显多于C组,差异均具有统计学意义(P<0.05);A组、B组和C组在Caspase-3中的Survivin mRNA表达量分别为0.938±0.041、0.983±0.057和0.388±0.076,A组、B组在Caspase-3中的Survivin mRNA表达量亦明显多于C组,差异均具有统计学意义(P<0.05)。结论 Survivin基因对肝癌细胞具有促进增殖和凋亡抑制的调节作用。

Objective To explore the expression and clinical significance of survivin gene in the proliferation and apoptosis of hepatocellular carcinoma cells.Methods HCC cells were divided into three groups:blank group(A group),vector virus group(B group)and survivin-siRNA lentivirus group(C group).MTT method was used to detect the proliferation inhibition rate of HepG2 cells;AV-PI apoptosis kit was used to detect the apoptosis rate of three groups of hepatoma cells;RT-qPCR was used to detect the relative expression of survivin and caspase-3.Results The proliferation rate of HepG2 cells in group A,B,and C were(7.38±0.422)%,(8.99±0.382)%,and(14.09±0.291)%,respectively,and the rates in group A and B were significantly lower than those in group C(P<0.05);the inhibition rates of apoptosis in group A,B,and C were(4.56±1.37)%,(3.05±1.08)%,and(39.45±12.88)%,respectively,and the rates in group A and B were significantly lower than that in group C(P<0.05);the relative expression of survivin gene detected by real-time PCR was 0.938±0.049,0.979±0.068 and 0.488±0.085 in group A,group B and group C,respectively,and the relative expression of survivin gene in group A and group B was significantly higher than that in group C(P<0.05);the expression of survivin mRNA in caspase-3 in group A,B,and C was 0.938±0.041,0.983±0.057,and 0.388±0.076,which was significantly higher in group A and B than in group C(P<0.05).Conclusion Survivin gene can promote the proliferation and inhibit the apoptosis of hepatoma cells.