低磷胁迫下玉米幼苗生理响应及相关基因表达研究

Physiological Response and Related Genes Expression of Maize Seedlings under Low Phosphorus Stress

以玉米品种合344为试验材料,对低磷胁迫下玉米幼苗叶片生理表型指标与玉米叶片转录组差异基因表达数据联合分析,探究低磷胁迫下与玉米幼苗叶片生理表型变化相关的关键响应基因及其调控规律。结果表明,低磷胁迫下玉米叶片的叶绿素荧光参数(FV/FM)以及叶绿素含量均有不同程度的降低,并且叶绿素合成相关基因大部分为下调表达,叶绿素降解相关基因大部分为上调表达。玉米叶片光合特性相关基因大部分呈下调表达,同时光合效率显著降低。在低磷胁迫下,SOD与POD活性随处理时间增长逐渐上升,编码两种酶的基因大部分上调表达。同时检测到8条MDA合成关键基因LOX,磷胁迫下其中7条均有不同程度的上调表达,同时MDA含量也随处理时间逐渐上升。

The maize variety 344 were used as the experimental material,and the physiological characteristics data and the transcriptome differential gene expression data of maize seedlings under low phosphorus stress were analyzed.To deciphering the molecular regulation of physiological changes in maize leaves under low phosphorus stress,and preliminaries identifiedkey genes involved in physiological response under low phosphorus stress.The results showed that under low phosphorus stress,chlorophyll fluorescence parameters(FV/FM)and chlorophyll content of maize leaves decreased in varying degrees,chlorophyll synthesis related genes were mostly down-regulated,and chlorophyll degradation related genes were mostly up-regulated.Most of the genes related to photosynthetic characteristics of maize leaves were down-regulated,and photosynthetic efficiency was significantly reduced.Under phosphorus stress,the activities of SOD and POD increased gradually with the increase in treatment time,and most of the genes encoding the two enzymes were up-regulated.At the same time,eight key genes of MDA synthesis LOX were detected,and seven of them were up-regulated under phosphorus stress.Meanwhile,the content of MDA increased gradually during low phosphorus treatment.