蒙药哈日-沙布嘎(铁杆蒿)的质量标准提升

Improvement of Quality Standard for Mongolian Medicine Artemisia sacrorum

目的:提升蒙药哈日-沙布嘎(铁杆蒿)的质量标准,为全面评价其质量提供科学依据。方法:对哈日-沙布嘎进行外观性状鉴别和显微鉴别;采用薄层色谱(TLC)法对药材中的东莨菪苷、绿原酸、咖啡酸、东莨菪内酯和3,5-二咖啡酰奎宁酸进行定性鉴别;建立高效液相色谱(HPLC)法测定药材中上述5种成分的含量;并对药材的水分、总灰分和浸出物进行检查。结果:哈日-沙布嘎药材茎呈类圆柱形,表面呈紫色或紫褐色或青褐色;叶呈卵形或矩圆状卵形,气香;花黄色,头状花序,近球形或半球形;粉末呈绿色或黄绿色,花粉粒具3个萌发孔,薄壁细胞簇晶棱尖锐,螺纹导管众多,偶见具缘纹孔导管,木纤维多成束存在。TLC结果显示,供试品在与5种对照品色谱相应位置上均显相同颜色的斑点。东莨菪苷、绿原酸、咖啡酸、东莨菪内酯、3,5-二咖啡酰奎宁酸的质量浓度分别在85.60~428.00、10.16~101.60、10.20~102.00、40.84~408.40、40.80~408.00μg/mL范围内与各自峰面积呈良好线性关系(r均大于0.9990);精密度、稳定性、重复性试验的RSD均小于3.00%(n=6);平均加样回收率分别为103.07%、99.66%、98.37%、97.78%、98.40%(RSD均小于3.00%,n=6)。10批药材样品中上述5种成分含量范围分别为0.36%~1.23%、0.09%~0.51%、0.04%~0.13%、0.61%~1.13%、0.12%~1.11%;水分、总灰分、水溶性浸出物的平均含量分别为6.25%、5.86%、26.50%。结论:该研究在哈日-沙布嘎原质量标准的基础上增加了显微鉴别、TLC鉴别、含量测定以及水分、总灰分和浸出物等检查项,方法精密、准确、稳定性良好,能够为更加科学、规范地评价该药材质量提供依据。

OBJECTIVE:To improve the quality standard of Mongolian medicine Artemisia sacrorum,and to provide scientific basis for comprehensive quality evaluation.METHODS:The appearance and microscopic characteristics of A.sacrorum were identified;scopoletin,chlorogenic acid,caffeic acid,scopoletin and 3,5-dicaffeoylquinic acid were identified quantitatively by TLC;the contents of above 5 components were determined by HPLC.The water content,total ash and extract were examined.RESULTS:The stem of A.sacrorum was cylindrical,and its surface was purple or purple-brown or cyan-brown;the leaves were ovate or oblong-ovate,fragrant;the flowers were yellow,head-shaped,subglobose or hemispherical.The powder was green or yellow-green,its pollen grain had three germination;the parenchymal cell clusters with sharp edges and numerous threaded ducts,occasionally having marginal pitted ducts;its wood fibers were in bundles mostly.Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for 5 substance control and samples.The linear range of scopoletin,chlorogenic acid,caffeic acid,scopolactone and 3,5-dicaffeoylquinic acid were 85.60-428.00,10.16-101.60,10.20-102.00,40.84-408.40 and 40.80-408.00μg/mL(all r>0.9990).RSDs of precision,stability,repeatability tests were all less than 3.00%(n=6).The average recoveries were 103.07%,99.66%,98.37%,97.78%,98.40%(all RSDs<3.00%,n=6).The contents of the above-mentioned 5 compounds in 10 batches of samples were 0.36%-1.23%,0.09%-0.51%,0.04%-0.13%,0.61%-1.13%,0.12%-1.11%,respectively;the average contents of water,total ash and water soluble extract were 6.25%,5.86%,26.50%,respectively.CONCLUSIONS:On the basis of the original quality standard of A.sacrorum,microscopic identification,TLC identification,content determination and examination items of water,total ash and extract are added.The method shows good precision,accuracy and stability,which can provide reference for more scientific and standardized evaluation of the quality of this medicinal material.