CAD基因在泛癌中的预后评估作用

Role of CAD gene in the prognosis evaluation of pan-cancer

目的探讨CAD基因在泛癌中的预后评估作用。方法从症基因组图谱数据库下载了33种肿瘤的转录组测序数据,同时下载了相应患者的临床信息、肿瘤突变负担和微卫星不稳定性的信息,使用log-rank检验与COX回归分析评估CAD基因表达与患者生存状况的关系,使用“ESTIMATE”法评估肿瘤微环境,使用“CIBERSORT”算法评估肿瘤免疫细胞浸润,使用Spearman相关评估CAD基因表达与靶标间的相关性。结果CAD基因在多种肿瘤组织中表达增高,并与不良预后相关。不同肿瘤中的结果具有很大程度上的一致性。CAD基因与多种肿瘤的肿瘤微环境密切相关。多种肿瘤的肿瘤突变负荷与微卫星不稳定相关。CAD基因与多种肿瘤的M0巨噬细胞浸润有关。结论CAD基因表达水平可以用于泛癌的预后评估,CAD基因高表达患者预后较差,需要制定个性化的治疗方案,加强护理与随访。

Objective To explore the prognostic role of CAD gene in the pan-cancer.Methods Transcriptome sequencing data of 33 cancers were downloaded from the cancer genome atlas database.The clinical information of the corresponding patients,tumor mutation burden and microsatellite instability information were also downloaded.The log-rank test and univariate COX regression were used to evaluate the survival.The"ESTIMATE"method was used to evaluate the tumor microenvironment,and the“CIBERSORT”algorithm was used to evaluate the tumor immune cell infiltration.Spearman correlation was used to evaluate the correlation between CAD gene expression and target.Results The expression of CAD gene was increased in a variety of tumor tissues and was related to poor prognosis.The results in different tumors are largely consistent.CAD gene was closely related to the tumor microenvironment of many tumors.The tumor mutation burden of many tumors was related to microsatellite instability.CAD gene expression was related to M0 macrophage infiltration in many tumors.Conclusion CAD gene can be used in the prognosis evaluation of pan-cancer,the patients with high CAD gene expression have a poor prognosis,and need a personalized treatment plan,strengthen care and follow-up.