高效液相色谱法测定蚁参蠲痹胶囊中丹参酮Ⅰ、隐丹参酮和丹参酮ⅡA的含量

Content determination of tanshinone Ⅰ, cryptotanshinone and tanshinoneⅡA in Yishen Juanbi Capsules by high performance liquid chromatography method

目的建立高效液相色谱法同时测定蚁参蠲痹胶囊中丹参酮Ⅰ、隐丹参酮和丹参酮ⅡA的含量。方法采用Agilent 5 TC-C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.02%磷酸(75∶25);检测波长为270 nm;体积流量为1.0 mL/min。结果丹参酮Ⅰ、隐丹参酮、丹参酮ⅡA分别在浓度为1.25~20.00μg/mL(r=0.9999)、5.05~80.78μg/mL(r=1.0000)、5.05~80.72μg/mL(r=0.9999)范围内,与各自峰面积呈现良好的线性关系,丹参酮Ⅰ、隐丹参酮和丹参酮ⅡA的平均加样回收率分别为97.01%、98.07%、95.48%,RSD分别为2.70%、2.62%、1.62%,符合要求。结论本法操作简单、结果可靠,为更好地控制蚁参蠲痹胶囊质量提供参考。

Objective To establish a high performance liquid chromatography method for the simultaneous content determination of tanshinoneⅠ,cryptotanshinone and tanshinoneⅡA in Yishen Juanbi Capsules.Methods The determination was performed on the column of Agilent 5 TC-C18(4.6 mm×250 mm,5μm)with mobile phase consisted of methanol-0.02%phosphoric acid(75∶25),flowing at 1.0 mL/min and the detection wavelength was set at 270 nm.Results TanshinoneⅠ,cryptotanshinone and tanshinoneⅡA showed good linear relationships in the ranges of 1.25-20.00μg/mL(r=0.9999),5.05-80.78μg/mL(r=1.0000),5.05-80.72μg/mL(r=0.9999),respectively,and the average recoveries were 97.01%,98.07%,95.48%and the RSD was 2.70%,2.62%,1.62%,respectively,which met the requirements.Conclusion This method is simple and accurate,and can be used for the quality control of Yishen Juanbi Capsules.