共载TPE衍生物和纳米银的PLGA复合载药体系的抑菌活性研究

TPE and Ag NPs Co-loaded PLGA Nanoparticles forHighly Effective Antibacterial Agent

通过复乳法合成复合纳米载药体系PLGA@TPE@Ag,其粒径大小300 nm左右,分散均一。其载药量达到了35±3.7%,包封率为73±0.9%,具有明显的药物缓释特性。PLGA@TPE@Ag对耐药性的大肠杆菌MIC值是2.3±0.09μg/mL。PLGA@TPE@Ag质量浓度为20μg/mL时对金黄色葡萄球菌和大肠杆菌的抑制率都高达90%以上。载药体系PLGA@TPE@Ag对细菌细胞内部形态的破坏可能是导致其细菌被抑制存活的主要机制。小鼠器官毒性试验明确PLGA@TPE@Ag无毒性。复合载药体系的抑菌活性相对于单一的载药体系有极大的提高。

The nano-drug carrier system PLGA@TPE@Ag was synthesized by the double emulsion method,with a particle size about 300 nm and uniform dispersion.The drug loading amount reached 35±3.7%;the encapsulation rate was 73±0.9%,which has obvious drug slow-release characteristics.The MIC value of PLGA@TPE@Ag to drug-resistant E.coli is 2.3±0.09μg/mL.The inhibition rate of PLGA@TPE@Ag at a concentration of 20μg/mL on the colony formation of Staphylococcus aureus and E.coli is as high as 90%.The destruction of bacterial cell morphology by PLGA@TPE@Ag may be the main antibacterial mechanism.The antibacterial activity of the PLGA@TPE@Ag is greatly improved compared with a single TPE and Ag NPs.