自身免疫性甲状腺炎小鼠的巨噬细胞极化失衡及代谢方式改变

Macrophage polarization imbalance and metabolic changes in autoimmune thyroiditis mice

目的探讨巨噬细胞极化失衡及其代谢途径改变在自身免疫性甲状腺炎(AIT)发生发展中的意义。方法将4周龄NOD.H-2h4雌鼠随机均分为对照组、AIT组(给予0.05%碘化钠水)。HE染色观察甲状腺组织淋巴细胞浸润情况并进行甲状腺炎性程度评分;ELISA测定血清甲状腺球蛋白抗体(TgAb);免疫荧光分析甲状腺组织中M1型和M2型巨噬细胞比例;细胞能量代谢分析仪(Seahorse)分析细胞能量代谢,测定细胞外酸化率(ECAR)、耗氧率(OCR)。结果AIT组甲状腺组织有明显的淋巴细胞浸润,AIT的发病率明显高于对照组(P<0.05)。与对照组相比,AIT组小鼠甲状腺组织中M1型巨噬细胞占比增多,M2型占比减少(P<0.05);AIT组小鼠的最大ECAR和OCR值均明显高与对照组(P<0.05)。结论巨噬细胞的极化失衡及其代谢改变对AIT的发生发展有着重要影响。

Objective To investigate the significance of macrophage polarization imbalance and metabolic pathway changes in the occurrence and development of autoimmune thyroiditis(AIT).Methods Four weeks old NOD.H-2h4 female mice were randomly divided into control group and AIT group(0.05%sodium iodide water).Hematoxylin-eosin(HE)staining was used to observe the lymphocyte infiltration in thyroid tissue.TgAb was measured by ELISA technology.The ratio of M1 type and M2 type macrophages in thyroid tissue was analyzed by immunofluorescence.Seahorse was used to analyze the energy metabolism of cells,and the extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)were measured.Results The thyroid tissue of mice in AIT group had obvious lymphocyte infiltration,and the incidence of AIT was significantly higher than the control group(P<0.05).Compared with the control group,the ratio of M1 and M2 macrophages in the thyroid tissue of mice in the AIT group increased,while the proportion of M2 type macrophages decreased(P<0.05);the ECAR and OCR values of mice in the high iodinedrinking water group were significantly higher than those of the control group(P<0.05).Conclusions The changes of macrophage metabolism and polarization imbalance affect the occurrence and development of AIT.