活血化瘀解毒法对OSF患者GSK-3β、β-连环蛋白水平的影响及作用机制的研究

Effect of Huoxue Huayu Detoxification on GSK-3βandβ-catenin levels in OSF patients and its mechanism

目的:探究活血化瘀解毒法对口腔黏膜下纤维化(oral submucous fibrosis,OSF)患者GSK-3β、β-连环蛋白(β-catenin)水平的影响及作用机制。方法:选取2018年5月~2019年12月本院收治的120例OSF患者,按照抽签法分为常规治疗组和活血化瘀解毒组各60例。常规治疗组使用曲安奈德注射液治疗,活血化瘀解毒组使用活血化瘀解毒法治疗。观察两组口腔黏膜病损面积、张口度、SFRP1在龈沟液及唾液中的水平、中医症候评分、TGF-β1、IL-6、GSK-3β、β-连环蛋白表达量及临床疗效。结果:治疗后活血化瘀解毒组口腔黏膜病损面积小于常规治疗组(P<0.05),活血化瘀解毒组张口度大于常规治疗组(P<0.05);治疗后活血化瘀解毒组患者SFRP1在龈沟液及唾液中的水平、临床疗效高于常规治疗组(P<0.05);治疗后活血化瘀解毒组中医症候评分、TGF-β1、IL-6、GSK-3β、β-连环蛋白表达量低于常规治疗组,差异均具有统计学意义(P<0.05)。结论:活血化瘀解毒法可通过作用于Wnt/β-catenin信号通路,调控TGF-β1、IL-6、GSK-3β、β-连环蛋白的表达,改善口腔黏膜下纤维化患者临床症状,缓解机体炎症,减轻疼痛,效果显著。

Objective:To explore the effects of Huoxue Huayu detoxification method on the levels of GSK-3βandβ-catenin in OSF patients and its mechanism.Methods:A total of 120 patients with oral submucosal fibrosis(OSF)treated in our hospital from May 2018 to December 2019 were selected and divided into routine treatment group and Huoxue Huayu detoxification group of 60 cases according to the lottery method.The routine treatment group was treated with triamcinolone acetonide injection,and the Huoxue Huayu detoxification group was treated with Huoxue Huayu detoxification.The lesion area,mouth opening degree,SFRP1 level in gingival crevicular fluid and saliva,TCM symptom score,TGF-β1,IL-6,GSK-3β,β-catenin expression and clinical effect were observed in the two groups.Results:After treatment,the lesion area of oral mucosa in the Huoxue Huayu detoxification group was smaller than that in the routine treatment group(P<0.05),and the mouth opening degree in the Huoxue Huayu detoxification group was larger than that in the routine treatment group(P<0.05);After treatment,the level of SFRP1 in gingival crevicular fluid and saliva and clinical effect in the Huoxue Huayu detoxification group were higher than those in the routine treatment group(P<0.05);the TCM syndrome score,TGF-β1,IL-6,GSK-3β,β-catenin expression in the Huoxue Huayu detoxification group were lower than those in the routine treatment group(P<0.05).Conclusion:By acting on Wnt/β-catenin signaling pathway,Huoxue Huayu detoxification method can regulate the expression of TGF-β1,IL-6,GSK-3β,β-catenin,improve the clinical symptoms of patients with oral submucous fibrosis,alleviate the body inflammation,alleviate the pain,with remarkable effect.