两株具有潜在益生作用的人源链球菌的安全性评价

Safety evaluation of two strains of human Streptococcus with potential probiotics

对分离于健康儿童口咽部的2株对金黄色葡萄球菌等常见致病菌具有广谱拮抗作用的甲型链球菌菌株1-4和4-2的安全性进行评价,为其作为呼吸道益生菌产品的研发和制备提供安全性依据。通过给予SPF级小鼠口服含有菌株1-4和4-2不同剂量的菌液的急性毒性实验以及耐药性的测定,观察动物是否出现死亡,急性毒性体征,细菌移位生长等情况。未发现动物死亡情况,实验组的体重变化采食量以及脏器指数均与对照组无显著差异,未有细菌移位生长的现象发生。菌株1-4和4-2均对9种常见抗生素的检测结果为敏感,其中菌株4-2对红霉素具有抗性。菌株1-4和4-2的安全性评价初步显示对小鼠无毒副作用,具有良好的安全性,可以作为呼吸道微生态制剂等产品的备用菌株。

The safety of strains 1-4 and 4-2,two potential probiotics isolated from the oropharynx of healthy children with broad-spectrum antagonism against Staphylococcus aureus and other common pathogenic bacteria,were evaluated so as to provide safety basis for the development and preparation of probiotics as respiratory tract products.The acute toxicity test and the determination of drug resistance of SPF mice with different doses of bacterial liquid containing strains 1-4 and 4-2 were conducted,and evaluation were made by observing death of the animals,signs of acute toxicity,bacterial translocation and growth,etc.Death of animals,or significant difference between the experimental group and the control group in weight change,food intake and organ index were not observed.Strains 1-4 and 4-2 were sensitive to 8 antibiotics tested and were resistant to cefotaxime and ceftriaxone,in addition strain 4-2 was also resistant to erythromycin.The safety evaluation of strains 1-4 and 4-2 showed that they were harmless to mice and had good safety,and could be used as backup strains for preparation of respiratory microecological and other products.