干扰素α对人骨肉瘤U2OS细胞化疗敏感性的影响

Effect of interferon-αon chemosensitivity in osteosarcoma U2OS cells

目的了解干扰素α(IFN-α)对骨肉瘤U2OS细胞化疗敏感性的影响。方法细胞分为对照组、IFN-α组、顺铂组和IFN-α+顺铂组,处理72 h,应用台盼蓝拒染法检测细胞生长抑制率,通过Hochest 33258荧光染色和DNA梯形条带检测凋亡,Caspase-3、8、9的表达由Western blot检测,细胞经Z-VAD-FMK预处理后,再使用顺铂单独或联合IFN-α处理,台盼蓝拒染法检测细胞生长抑制率。结果IFN-α组、顺铂组、IFN-α+顺铂组细胞生长抑制率均高于对照组,IFN-α+顺铂组细胞生长抑制率高于顺铂组(P<0.01)。IFN-α+顺铂组出现DNA梯形条带。与顺铂组比较,IFN-α+顺铂组Caspase-3、8、9活性片段的表达增加,差异有统计学意义(P<0.01)。IFN-α+顺铂+Z-VAD-FMK组细胞生长抑制率低于IFN-α+顺铂组(P<0.01)。结论IFN-α通过Caspase依赖性凋亡增强U2OS细胞对顺铂的化疗敏感性。

Objective To study the effect of interferon-α (IFN-α) on chemosensitivity in osteosarcoma U2OS cells. Methods Cells were divided into control group, IFN-α group, Cisplatin group and IFN-α+Cisplatin group. After treatment for 72 hours, cell growth inhibition rate was determined by Trypan Blue exclude test. Apoptosis was evaluated with Hoechst 33258 staining and DNA ladder. Expressions of caspase-3, 8 and 9 were detected through Western blot. After pre-treatment with Z-VAD-FMK, cell was treated with Cisplatin or combination. Growth inhibition was determined with Trypan Blue exclude test. Results Cells growth inhibition rates in IFN-α group, Cisplatin group and IFN-α+Cisplatin group were higher than those in control group, cells growth inhibition rate in IFN-α+Cisplatin group was higher than that in Cisplatin group (P < 0.01). DNA ladder appeared in IFN-α+Cisplatin group. Compared with Cisplatin group, the expression of caspase-3, 8 and 9 in IFN-α+Cisplatin group was significantly increased (P < 0.01). Cells growth inhibition rate of IFN-α+Cisplatin+Z-VAD-FMK group was lower than that of IFN-α+Cisplatin group (P < 0.01). Conclusion IFN-α enhances chemosensitivity to Cisplatin in U2OS cells through caspase-dependent apoptosis.