基因芯片技术检测Shh蛋白对脑梗死小鼠脑皮质组织microRNA-210表达的影响

Effect of Shh protein on cortical microRNA-210 expression in cerebral infarction by gene chip technology

目的探讨Shh蛋白对脑梗死小鼠脑皮质组织microRNA-210表达的影响。方法建立小鼠脑皮质梗死模型,基因芯片技术筛选出脑梗死小鼠脑皮质组织差异表达基因,并应用qRT-PCR技术对其差异性表达进行验证。给予Shh蛋白治疗后,应用qRT-PCR技术观察Shh蛋白对脑梗死小鼠脑皮质组织microRNA-210表达。应用ATP试剂盒检测Shh蛋白对脑梗死小鼠脑皮质组织ATP活性的变化。结果与对照组相比,基因芯片筛选出脑梗死小鼠脑皮质组织中差异表达microRNA 17个,其中表达上调8个,表达下调9个。进一步筛选出microRNA-210,运用q-PCR技术验证其在脑梗死后脑皮质组织中表达上调(P<0.05)。与脑梗死组相比较,经Shh蛋白干预后小鼠皮质组织microRNA-210表达上调(P<0.05)与对照组相比,脑梗死组ATP含量下降,Shh蛋白干预后,ATP含量有所上升(P<0.05)。结论Shh蛋白可能通过上调microRNA-210逆转能量生成障碍对脑梗死发挥治疗作用。

Objective To investigate the effect of Shh protein on microRNA-210 expression in cerebral cortex of mice with cerebral infarction. Method A cerebral cortex infarction mouse model was established. The differentially expressed genes in the cerebral cortex of the mice with cerebral infarction were screened by gene chip technology, and the differentially expressed genes were verified by qRT-PCR. After treatment with Shh protein, qRTPCR was used to observe the expression of Shh protein on microRNA-210 in cerebral cortex of mice with cerebral infarction. ATP kit was used to detect the changes of Shh protein on ATP activity in cerebral cortex of mice with cerebral infarction. Results Compared with the Sham group, 17 microRNA differentially expressed in the cerebral cortex of the cerebral infarction mice were screened by gene chip, among which 8 microRNA were up-regulated and 9 microRNA were down-regulated. MicroRNA-210 was further screened and qRT-PCR was used to verify its upregulated expression in cerebral cortex after cerebral infarction(P< 0.05). Compared with the cerebral infarction group, microRNA-210 expression in the cortical tissue of mice was up-regulated after Shh protein intervention(P< 0.05). Compared with the Sham group, the ATP content in the cerebral infarction group decreased, while increased after Shh protein treatment(P< 0.05). Conclusion Shh protein may play a therapeutic role in cerebral infarction by upregulating microRNA-210 to reverse energy generation.