ARTP诱变结合抗性筛选选育西索米星高产菌株

Breeding of high-yield sisomicin strain through the combination of ARTP mutagenesis and resistance screening

目的利用等离子体诱变,结合抗生素抗性筛选,获得西索米星高产菌株。方法首先通过链霉素抗性筛选,获得一株比出发菌株产抗能力高的S4;随后以S4为出发菌株,经等离子体处理40s,借助巴龙霉素抗性筛选,得到双重抗性菌株。最后对突变株进行再次诱变,并结合高浓度链霉素抗性筛选。结果获得一株高产突变株M. inyoensis SAAP22,比出发菌株效价提高了38.18%,具有稳定的遗传性能。结论通过等离子体诱变,结合抗生素抗性筛选,可有效提升伊尼奥小单孢菌的西索米星合成能力,所得高产菌株具有潜在应用价值。

Objective Atmospheric and room temperature plasma(ARTP) mutagenesis combined with antibiotic resistance screening was used to obtain high-yield sisomicin strains. Methods First, we obtained a S4 strain with a higher yield than the starting strain, which was obtained through streptomycin resistance screening. Then we used S4 as the starting strain and treated S4 with atmospheric and room temperature plasma(ARTP) for 40 s. Then the strains were screened for resistance with streptomycin paromomycin and ampicillin, and dual resistance was introduced step by step. Finally, the positive mutants were mutagenized again and combined with high concentration streptomycin resistance screening. Results The strain of Micromonospora inyoensis SAAP22 with high sisomicin production was obtained. The titer was 38.18% higher than the original strain and it has stable genetic property. Conclusion The method of using atmospheric and room temperature plasma(ARTP) mutagenesis combined with antibiotic resistance screening can effectively improve the ability of Micromonospora inyoensis to produce sisomicin. The Micromonospora inyoensis strain SAAP22 has potential application value to produce sisomicin.