胎儿单纯性肠管强回声与肠道菌群的关系及其临床意义

Relationship between fetal echogenic bowel and intestinal flora and its clinical significances

目的观察中孕期存在肠管强回声(EB)胎儿与正常胎儿分娩后新生儿肠道菌群差异,探讨EB与肠道菌群的关系及其临床意义。方法对中孕期产前超声检查无异常的13胎胎儿(正常组)和7胎EB胎儿(EB组)采集出生后首次胎便,行16S rRNA测序。结果测序后共获得539988个运算分类单元(OTU)。EB组与正常组肠道菌群多样性、群落组成结构差异均无统计学意义(P均>0.05)。门水平上,EB组厚壁菌门(Firmicutes)和克雷伯菌门(Klebsiella)丰度均显著高于正常组(P均<0.05),梭杆菌门(Fusobacteria)丰度显著低于正常组(P<0.05)。属水平上,EB组葡萄球菌属(Staphylococcus)和弯曲杆菌属(Campylobacter)丰度均显著高于正常组(P均<0.05),埃希菌/志贺菌属(Escherichia/Shigella)、拟杆菌属(Bacterium)、梭菌属(Clostridium)及肠杆菌属(Enterobacter)丰度均显著低于正常组(P均<0.05)。结论EB组与正常组胎儿出生后肠道菌群多样性、菌群群落组成结构无明显差异,但肠道菌群相对丰度存在差异,提示肠道菌群失调可能是导致EB形成的原因之一。

Objective To eobserve the difference of intestinal flora between fetuses with echogenic bowel(EB)and normal fetuses after delivery,in order to explore the relationship between fetal EB and intestinal flora and its clinical significances.Methods Thirteen normal fetuses showed no abnormality with prenatal ultrasound(normal group)and 7 fetuses with EB(EB group)were selected.After delivery,the first fetal stool was collected for 16S rRNA sequencing.Results A total of 539988 operational taxonomic units(OTU)were obtained from all samples after sequencing.There was no significant difference of intestinal flora diversity nor community composition between EB group and normal group(both P>0.05).At phylum level,the abundance of Firmicutes and Klebsiella in EB group was significantly higher than those in normal group(both P<0.05),and of Fusobacteria in EB group was significantly lower than that in normal group(P<0.05).At generic level,the abundance of Staphylococcus and Campylobacter in EB group were significantly higher than those in normal group(both P<0.05),while of Escherichia/Shigella,Bacterium,Clostridium and Enterobacter in EB group were significantly lower than those in normal group(all P<0.05).Conclusion There was no significant difference of intestinal flora diversity nor composition between EB group and normal group,but the relative abundance of intestinal flora was different between groups,indicating that intestinal flora imbalance may be one of the factors leading EB.