烟草甲对不同波长光源的趋性及视蛋白序列分析

Phototaxis to different wavelength light sources and opsin sequences analysis of Lasioderma serricorne(Fabricius)

为明确烟草甲(Lasioderma serricorne)的趋光行为特性,通过室内和实仓诱捕试验比较了烟草甲对不同波长光源诱虫灯的趋性差异,并通过转录组测序鉴定了烟草甲视蛋白基因序列。结果表明,烟草甲对不同波长光源的趋性存在差异。烟草甲对400~405 nm波长光源的趋性最强,对600~605 nm波长的趋性次之。实仓诱捕试验结果表明,400 nm波长的诱虫灯对烟草甲的诱集数量显著高于380 nm的诱虫灯。烟草甲有两个与敏感光吸收相关的视蛋白,分别为UV和LW视蛋白,且烟草甲对400~405 nm和600~605 nm波长的趋光行为与UV和LW视蛋白对波长的敏感范围一致,烟草甲缺失BL视蛋白。因此,400 nm波长的诱虫灯可用于实仓中烟草甲的诱杀防治。

In order to ascertain the phototaxis behavior characteristics of Lasioderma serricorne,this study compared the phototaxis of L.serricorne to light traps with different wavelength light sources through laboratory and warehouse trapping tests,and identified the sequences of opsin gene by RNA-seq.The results showed that L.serricorne had different phototaxis to light sources of different wavelengths,its phototaxis to 400-405 nm was the strongest,followed by 600-605 nm.The results of warehouse trapping tests showed that the amount of L.serricorne trapped by 400 nm light was significantly more than that by 380 nm light.L.serricorne had two opsins associated with sensitive light wavelength absorption,UV and LW.The phototaxis behaviors of L.serricorne to 400-405 nm and 600-605 nm were in accordance with the sensitivity ranges of UV opsin and LW opsin respectively.L.serricorne lacked BL opsin.Therefore,400 nm wavelength light could be used to control L.serricorne in warehouses.