凝结芽孢杆菌13002产芽孢条件优化

Optimizing Spore-Forming Conditions of Bacillus coagulans 13002

通过单因素实验及Box-Behnken响应面法优化凝结芽孢杆菌13002产芽孢的培养基配方与培养条件进行优化,以获得高芽孢数。结果表明:当培养基配方为葡萄糖15.0 g,细菌学蛋白胨10.0 g,酵母提取粉20.0 g,NaCl 10.0 g,MnSO_(4)0.2 g,K_(2)HPO_(4)3.0 g,并用10%的麸皮水浸提液定容至1 L;培养条件为初始pH7.0、接种量6%(V/V)、装液量30 mL(250 mL锥形瓶)、于200 r/min、40℃培养52 h时,获得的芽孢数可达3.31×10^(9) CFU/mL。本试验为工业化生产凝结芽孢杆菌13002制剂奠定基础。

In this study,the highest spore number was obtained by single factor experiment and box-behnken response to the medium formulation and culture conditions for producing spores of Bacillus coagulans 13002.Finally,the optimal medium formula was determined as glucose 15.0 g,bacteriology peptone 10.0 g,yeast extract 20.0 g,NaCl 10.0 g,MnSO_(4)0.2 g,K_(2)HPO_(4)3.0 g,and 10%bran water extract to 1 L.The optimal culture conditions were the initial pH value of 7.0,inoculation amount of 6%(V/V),liquid loading amount of 30 mL(250 mL conical flask),and culture at 200 r/min at 40℃for 52 h.The final spore number was 3.31×10^(9) CFU/mL.This study will lay down a foundation for industrial scale production of Bacillus coagulans 13002.