铁氨基黏土-葡萄糖氧化酶纳米复合催化剂的构筑及多酶级联反应研究

Construction of Fe-aminoclay-glucose Oxidase Nanocomposite Catalyst and Its Multi-enzyme Cascade Analysis

以铁氨基黏土(FeAC)为载体,通过共价交联固定葡萄糖氧化酶(GO_(x)),构筑了铁氨基黏土-葡萄糖氧化酶纳米复合催化剂(FeAC-GO_(x)).利用FeAC的过氧化物酶活性,与GO_(x)结合进行级联反应,可催化葡萄糖转化为过氧化氢并产生显色反应;采用扫描电子显微镜(SEM)、X射线衍射(XRD)和傅里叶变换红外光谱(FTIR)对FeACGO_(x)进行了形貌和结构表征,并评价了其酶动力学参数、催化稳定性和重复使用性等.结果表明,GO_(x)的固定化率可达到76.4%,所构筑的纳米结构酶复合体系具有高效的级联催化能力.与天然酶体系相比,FeAC-GO_(x)具有更优异的温度和pH耐受性,且在重复使用6次后,酶催化活性无明显降低.该体系不仅为新型葡萄糖传感器的开发奠定了基础,还为多酶级联纳米结构酶的构筑提供了新思路.

Glucose oxidase(GO_(x))and Fe-aminoclay(FeAC)were combined to construct a mimic multienzyme system(FeAC-GO_(x))for cascade reaction.FeAC exhibit peroxidase-like activity which can catalyze H2O2 generated by GO_(x) and trigger color reaction.The obtained FeAC-GO_(x) was characterized by scanning elec⁃tron microscope(SEM),X-ray diffraction(XRD),and fourier transform infrared spectroscopy(FTIR).The en⁃zymatic kinetics,catalytic stability and reusability were evaluated.As a result,the immobilization efficiency of GO_(x) could reach to 76.4%,and FeAC-GO_(x) exhibited excellent cascade catalysis activity.Compared with the free enzyme,FeAC not only possessed a strong tolerance toward temperature and pH,but also presented excel⁃lent reusability.The catalytic activity of reused FeAC barely changed after six repetitions.This strategy may lay the foundation to the development of a new glucose biosensor,and provide a novel approach to design multi-enzyme cascade system.