富硒当归淫羊藿方对大鼠睾丸SOD、MDA及血清性激素的影响

Effects of Selenium-enriched Danggui Yinyanghuo Recipe on SOD,MDA and serum sex hormone in rat testis

目的研究不同剂量的富硒当归淫羊藿方对大鼠睾丸超氧化物歧化酶(SOD)、丙二醛(MDA)及血清性激素[卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)]的影响。方法随机选取10只雄性SD大鼠作为正常组(灌胃生理盐水),余下大鼠按照随机数字表法分为四组:模型对照组(灌胃腺嘌呤)、富硒当归淫藿方高剂量组[灌胃腺嘌呤+15 d后灌胃20.92 g/(kg·d)富硒中药]、中剂量组[灌胃腺嘌呤+15 d后灌胃10.46 g/(kg·d)富硒中药]、低剂量组[灌胃腺嘌呤+15 d后灌胃5.23 g/(kg·d)富硒中药],每组各12只,实验共45 d。检测并比较各组大鼠一般状态、睾丸SOD和MDA、血清性激素及睾丸形态学变化。结果一般状态观察:造模第4天开始,模型对照组与富硒当归淫羊藿方各剂量组均出现饮食量减少及体重下降、尿量增多的临床表现。开始实施药物干预后,富硒当归淫羊藿方低、中、高剂量组饮食量及体重逐渐增加,尿量等临床表现逐渐趋于正常。与正常组比较,模型对照组睾丸组织匀浆SOD下降,MDA上升,差异均有高度统计学意义(均P<0.01);与模型对照组比较,富硒当归淫羊藿方高、中剂量组SOD均不同程度升高,差异均有高度统计学意义(均P<0.01),但富硒当归淫羊藿方低剂量组与模型对照组比较,差异无统计学意义(P>0.05);与模型对照组比较,富硒当归淫羊藿方高、中、低剂量组MDA均不同程度降低,差异均有高度统计学意义(均P<0.01)。与正常组比较,模型对照组血清FSH、LH升高,血清T降低,差异均有高度统计学意义(均P<0.01)。与模型对照组比较,富硒当归淫羊藿方高、中剂量组血清FSH、LH降低,血清T升高,差异均有高度统计学意义(均P<0.01)。睾丸组织病理显示:模型对照组可见明显的睾丸损伤,富硒当归淫羊藿方低、中、高剂量组均有改善。结论富硒当归淫羊藿方可改善腺嘌呤造模后大鼠睾丸SOD、MDA及血清性激素的水平,对腺嘌呤导致的睾丸损伤有一定的治疗作用。

Objective To study the effects of different doses of Selenium-enriched Danggui Yinyanghuo Recipe on superoxide dismutase(SOD),malondialdehyde(MDA)and serum sex hormones(follicle stimulating hormone[FSH],luteinizing hormone[LH]and testosterone[T])in rats testis.Methods Ten male SD rats were randomly selected as the normal group(gavage physiological saline),and the remaining rats were divided into four groups according to random number table method:model control group(gavage adenine),Selenium-enriched Danggui Yinyanghuo Recipe high dose group(gavage adenine+15 days later,gavage 20.92 g/[kg·d]selenium-enriched traditional Chinese medicine),middle dose group(gavage adenine+15 days later,gavage 10.46 g/[kg·d]selenium-enriched traditional Chinese medicine),low dose group(gavage adenine+15 days later,gavage 5.23 g/[kg·d]selenium-enriched traditional Chinese medicine).The experiment lasted for 45 days with 12 rats in each group.The general state,SOD and MDA of testis,serum sex hormone and testicular morphology of rats were detected and compared in each group.Results Observation of general state:from the fourth day of modeling,the model control group and the Selenium-enriched Danggui Yinyanghuo Recipe groups showed clinical manifestations of reduced diet,decreased body weight and increased urine volume.After the implementation of drug intervention,the diet and body weight of the low,medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe gradually increased,and the urine volume and other clinical manifestations gradually tended to be normal.Compared with normal group,SOD decreased and MDA increased in testicular homogenate of model control group,with high statistical significance(all P<0.01).Compared with the model control group,SOD in the Selenium-enriched Danggui Yinyanghuo Recipe high dose and medium dose groups increased to varying degrees,with high statistical significance(all P<0.01).However,there was no significant difference between Selenium-enriched Danggui Yinyanghuo Recipe low dose group and model control group(P>0.05).Compared with the model control group,MDA in the high,medium and low dose groups of Selenium-enriched Danggui Yinyanghuo Recipe was decreased to different degrees,with high statistical significance(all P<0.01).Compared with normal group,serum FSH and LH were increased in model control group,while serum T was decreased,with high statistical significance(all P<0.01).Compared with model control group,serum FSH and LH were decreased and serum T was increased in the high and medium dose groups of Selenium-enriched Danggui Yinyanghuo Recipe,with high statistical significance(all P<0.01).Histopathology of testis showed obvious testicular injury in model control group,and improvement in low,medium and high dose groups of Selenium-enriched Danggui Yinyanghuo Recipe.Conclusion Selenium-enriched Danggui Yinyanghuo Recipe can improve the levels of SOD,MDA and serum sex hormones in the testis of rats after adenine modeling,and has a certain therapeutic effect on the testis injury caused by adenine.