2种儿童肺炎支原体感染检测方法的比较与分析

The comparison and analysis of two detection methods for Mycoplasma pneumoniae infection in children

目的分析被动凝集法和胶体金法在儿童肺炎支原体感染中的检测效果,比较2种检测方法的临床应用价值。方法收集于杭州师范大学附属医院就诊的呼吸道感染患儿247例,经过临床确诊分为支气管炎组(84例)、上呼吸道感染组(80例)、肺炎组(70例)、哮喘组(7例)和发热组(6例)。在入院初收集患儿的血清和咽拭子,分别采用被动凝集法和胶体金法进行肺炎支原体检测,比较2种方法在不同呼吸道感染病例组中的阳性检出率。结果在247例标本的检测中,被动凝集法的阳性率(73.68%)高于胶体金法(26.72%),差异有统计学意义(P <0.05)。经配对资料χ^(2)检验,被动凝集法在支气管炎组、上呼吸道感染组、肺炎组、哮喘组和发热组的阳性率均高于胶体金法,差异有统计学意义(P <0.05)。结论相比于胶体金法,被动凝集法在各种儿童呼吸道感染疾病中的检出效果更佳,临床诊断价值更佳明显,在今后的支原体感染诊断中可加大被动凝集法的检测力度,为临床及时和准确诊断提供依据。

Objective To analyze the detection effect of passive agglutination method and colloidal gold method in children with mycoplasma pneumoniae infection,and to compare the clinical application value of the two detections. Methods Totally,247 cases of respiratory tract infection were collected from Affiliated Hospital of Hangzhou Normal University,and they were divided into bronchitis group( 84 cases),upper respiratory tract infection group( 80 cases),pneumonia group( 70 cases),asthma group( 7 cases) and fever group( 6 cases) after clinical diagnosis. Serum and throat swabs were collected before admission,and the positive rates of the two methods were compared in different respiratory infection groups. Results The results showed that in 247 samples,the positive rate of passive agglutination method( 73. 68%) was higher than that of colloidal gold method( 26. 72%),the difference was statistically significant( P < 0. 05). The positive rate of passive agglutination in bronchitis group,upper respiratory tract infection group,pneumonia group,asthma group and fever group was higher than that of colloidal gold method by χ^(2) test,with the differences statistically significant( P < 0. 05). Conclusion Compared with colloidal gold method,passive agglutination method has better detection effect and clinical diagnosis value in all kinds of children’s respiratory tract infections. In the future diagnosis of mycoplasma infection,passive agglutination method can be strengthened to provide basis for clinical timely and accurate diagnosis.