摘要
目的探索肿瘤源性免疫球蛋白G(IgG)表达对胃癌细胞增殖的影响和Na^(+)-K^(+)-ATP酶在其中所扮演的角色。方法用siRNA干扰技术下调胃癌细胞SGC-7901和HGC-27中IgG表达后:①用免疫印迹和RT-qPCR技术在蛋白水平和mRNA水平检测IgG表达情况;②分别用CCK-8法和镜下细胞拍照计数法检测以上胃癌细胞的增殖能力;③建立稳定表达Control shRNA和IGHG1 shRNA的HGC-27胃癌细胞系,完成裸鼠成瘤实验;④用比色法检测以上细胞中总ATP酶和Na^(+)-K^(+)-ATP酶的活性。结果体内外实验表明,IgG表达下调使胃癌细胞增殖受到抑制,胃癌细胞中总ATP酶和Na^(+)-K^(+)-ATP酶活性显著降低(P<0.05)。结论肿瘤源性IgG可能通过增强Na^(+)-K^(+)-ATP酶活性促进胃癌细胞增殖。
Objective To explore the effect of tumor-derived immunoglobulin G(IgG)expression on the proliferation of gastric cancer cells and the role of Na^(+)-K^(+)-ATPase.Methods After the siRNA interference was used to down-regulate IgG expression in gastric cancer cells SGC-7901 and HGC-27.①Western blotting and RT-qPCR were used to detect the expression of IgG at the protein and mRNA levels.②CCK-8 method and microscopic cell photo-counting were used to detect the proliferation ability of the above gastric cancer cells.③Gastric cancer cell lines HGC-27 with stable expressions of Control shRNA and IGHG1 shRNA was established to complete the tumorigenesis experiment in nude mice.④The activity of total ATPase and Na^(+)-K^(+)-ATPase in the above cells were detected by colorimetry.Results In vivo and in vitro experiments showed that down-regulation of IgG expression inhibited the proliferation of gastric cancer cells,and the activities of total ATPase and Na^(+)-K^(+)-ATPase in gastric cancer cells significantly decreased(P<0.05).Conclusion Tumor-derived IgG may promote the proliferation of gastric cancer cells by enhancing Na^(+)-K^(+)-ATPase activity.
作者
王居平
赵静
周青宏
巫祥生
农安娜
华浩铭
杨树琳
陈丽莹
任瑞华
彭慧
Wang Juping;Zhao Jing;Zhou Qinghong;Wu Xiangsheng;Nong Anna;Hua Haoming;Yang Shulin;Chen Liying;Ren Ruihua;Peng Hui(Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Gannan Medical University,Ganzhou 341000,Jiangxi,China)
出处
《右江民族医学院学报》
2021年第1期1-5,16,共6页
Journal of Youjiang Medical University for Nationalities
基金
国家自然科学基金项目(81760513、82060528)
广西自然科学基金项目(2017JJA10351、2019JJA140532)
广西高校中青年教师基础能力提升项目(2017KY0520)
广西高等学校重点实验室科学研究开放课题(kfkt2017005)
广西学术学位硕士研究生创新课题(YCSW2020232)
广西壮族自治区区级大学生创新创业训练计划项目(201810599043、201810599036、202010599011、202010599025)
右江民族医学院校级科研项目(yy2016bsky02)。