基于网络药理学的黄精丸干预糖脂代谢紊乱机制研究

Mechanism of Huangjing Pill on improving disorder of glycolipid metabolism based on network pharmacology

目的:探究黄精丸干预糖脂代谢紊乱的机制。方法:从中医药百科全书(ETCM)等数据库和相关文献搜集成分,根据胃肠吸收、P-糖蛋白底物和成药性模拟筛选活性成分,从SwissTargetPrediction等数据库搜集靶点,使用Cytoscape软件构建成分-靶点网络,并进行蛋白相互作用网络分析、GO功能富集和KEGG通路分析。结果:甾体皂苷类是黄精丸的主要活性成分,作用于由磷脂酰肌醇-3-激酶(PI3K)、信号转导和转录激活因子3(STAT3)等69个靶点构成的蛋白网络。靶点富集成蛋白质丝氨酸/苏氨酸激酶活动、PI3K-蛋白激酶B(PI3K-AKT)等通路。结论:网络药理学初步表明黄精丸调节糖脂代谢可能是通过甾体皂苷类成分,影响PI3K和STAT3等靶点,进一步影响蛋白质丝氨酸/苏氨酸活动、PI3K-AKT信号等通路。

Objective: To investigate the mechanism of Huangjing Pill on improving the disorder of glycolipid metabolism. Methods: Components were obtained from databases such as ETCM and related literatures. GI-absorption, P-gp substrate and drug-likeness were employed to screen out the active components. Databases such as SwissTargetPrediction were used to predict targets. Cytoscape software was used to establish component-target network, and the protein-protein interaction network analysis, GO function enrichment and KEGG pathway analysis were performed. Results: Steroidal saponins were the main active ingredients of Huangjing Pill, acting on the protein network composed of 69 targets including phosphatidylinositol-3-kinase(PI3 K), signal transducer and activator of transcription 3(STAT3)and so on. These targets were significantly enriched into protein serine/threonine kinase activity, phosphatidylinositol-3-kinase-protein kinase B(PI3 K-AKT)signaling pathway and other pathways. Conclusion: Network pharmacology reveals that steroidal saponins in Huangjing Pill play a role in glycolipid metabolism by influencing PI3 K, STAT3 and other targets as well as protein serine/threonine activity and PI3 K-AKT signal pathway.