不同月份产羔母羊泌乳期生殖激素与生长激素监测

Detection of reproductive hormone and growth hormone of ewes giving births in different months

为了解不同月份生产的奶山羊泌乳期生殖激素与生长激素(GH)变化规律,随机抽取1、3、5和8月份分娩的奶山羊各10只,于分娩后0 d、7 d、1~8个月(每月的第15天)时采集母羊静脉血,分离血清,采用ELISA试剂盒检测母羊外周血中催乳素(PRL)、卵泡刺激素(FSH)、促黄体生成素(LH)、雌激素(E_(2))、孕酮(P_(4))和GH水平变化。结果显示:不同月份产羔母羊泌乳期间外周血中同一激素的动态变化趋势一致;在整个泌乳期间,1、3、5、8月份产羔母羊PRL、FSH、LH、P_(4)、E_(2)、GH含量的动态变化范围分别为446.17~221.72 ng·L^(-1)、9.49~3.82 U·L^(-1)、351.17~218.16 pg·mL^(-1)、4086.83~3568.15 pmol·L^(-1),33.74~22.30 ng·L^(-1)、30.36~11.57μg·L^(-1);不同月份产羔母羊外周血中GH水平在相同泌乳期均无显著差异(P>0.05),FSH水平在0 d有显著差异(P<0.05),P_(4)水平在8个月时有显著差异(P<0.05),PRL水平在泌乳的0 d和2、3、8个月时有显著差异(P<0.05),LH水平在泌乳的0 d、2个月时有显著差异(P<0.05),E_(2)水平在泌乳0 d、3个月和8个月时有显著差异(P<0.05)。结果表明:奶山羊产羔月份的不同对泌乳期间的激素水平有一定的影响,但动态变化趋势一致。

To understand the changes in reproductive hormones and growth hormones of milk goats during lactation in different months,ten samples of each group of the ewes kidding in January,March,May and July were randomly selected,respectively,and blood samples were collected from them on days 0 and 7 and on the 15th day of each month from the first to 8th months after lactation.The serum samples were isolated by centrifugation,and their levels of prolactin(PRL),follicle-stimulating hormone(FSH),luteinizing hormone(LH),progesterone(P_(4)),Estrogen(E_(2)),and growth hormone(GH)were measured using an ELISA kit.The results showed that the dynamic trend of the same hormones of the ewes lambing in different months were consistent during the whole lactation period.The range of PRL,FSH,LH,P_(4),E_(2),and GH changes in the peripheral blood of the animals were 446.17-221.72 ng·mL^(-1),9.49-3.82 U·L^(-1),351.17-218.16 pg·mL^(-1),4086.83-3568.15 pmol·L^(-1),33.74-22.30 ng·L^(-1),30.36-11.57μg·L^(-1),respectively.In the peripheral blood of the ewes kidding in different months in the same lactation period,there were no significant differences in the GH content,but there were significant differences in the FSH content only at 0 d and in P_(4)only in the 8th month of lactation.The PRL content was significantly different at 0 d,in the 2nd,3rd and 8th months of lactation(P<0.05),the LH content was significantly different at 0 d and in the 2nd month of lactation(P<0.05),and the E_(2)content was significantly different at 0 d,in the 3rd and 8th months of lactation(P<0.05).The above results indicated that kidding in different months had a certain effect on the hormone levels of the ewes during lactation,but their dynamic trends tended to be consistent.