硝化应激在神经型犬瘟热疾病发展中的临床意义

Clinical significance of nitration stress in the development of canine distemper

为了研究硝化应激在神经型犬瘟热疾病发展中的作用,并探讨其对该病的诊断价值,选取犬瘟热临床病例113例,其中有神经症状组(NCD)共37例,无神经症状组(NNS)共76例,并设25例无犬瘟热健康犬作为对照组(CG)。就诊时分别采取血液和脑脊液,测定一氧化氮(NO)、神经元型一氧化氮合成酶(nNOS)和神经元特异性烯醇化酶(NSE)水平。结果显示:NCD组2种组织液中的NO、nNOS和NSE升高,显著高于NNS组(P<0.05)与CG组(P<0.01);ROC曲线分析发现,2种组织液中的NO、nNOS和NSE对神经型犬瘟热的诊断具有良好的灵敏度和特异性,血浆中以nNOS最佳,截断值为230.1 ng/mL,脑脊液中以NSE最佳,截断值为3.67 ng/mL;NSE对神经型犬瘟热具有一定预后价值,血浆中NSE<2.07的病例死亡率显著低于NSE≥2.07的病例。结果表明:神经型犬瘟热疾病发展中伴有硝化应激和神经损伤,血浆与脑脊液中的NO、nNOS和NSE具有对神经型犬瘟热的诊断及预测作用。

The purpose of this study was to investigate the role of nitration stress in the development of canine distemper(CD) and to elaborate its diagnostic value for CD. 113 clinical cases of CD were included in the study, which were divided into the group with neurological symptoms(the NCD group, 37 cases in total) and the group without neurological symptoms(the NNS group, 76 cases in total). In addition, 25 healthy dogs without CD were selected as the control group(the CG group). The levels of nitric oxide(NO), neuronal nitric oxide synthase(nNOS) and Neuron specific enolase(NSE) were measured in the plasma and cerebrospinal fluid(CSF) of the animals at visit. The results showed that the levels of NO, nNOS and NSE in both the plasma and CSF of the NCD group were higher than those of the NNS group(P<0.05) and the CG group(P<0.01). ROC curve analysis showed that NO, nNOS and NSE in both the plasma and CSF of the animals had good sensitivity and specificity for the diagnosis of CD. In the plasma samples, nNOS was the best biochemical markers, and its critical value was 230.1 ng/mL. NSE was the best biochemical markers in CSF, and its critical value was 3.67 ng/mL. The level of NSE had certain prognostic value for canine distemper. The mortality of the patients with NSE<2.07 in plasma was significantly lower than those of the patients with NSE ≥2.07. In summary, nitration stress and nerve injury occurred in the development of canine distemper. The levels of NO, nNOS and NSE in plasma and CSF were of value in diagnosing and predicting canine distemper.