斑纹光躄鱼5种组织中脂肪酸成分

Analysis of fatty acid composition in five tissues of Histrio histrio

目的分析斑纹光躄鱼胸肌、鳍肌、胃、皮肤及骨骼中脂肪酸组成。方法先用5%硫酸-甲醇溶液将5种组织中脂肪酸甲酯化,再用气相色谱法测定脂肪酸含量。结果 5种组织中饱和、单不饱和、多不饱和脂肪酸含量分别为42.03%~47.68%、13.63%~20.37%、29.53%~41.29%。胸肌组织中多不饱和脂肪酸含量最高,骨组织中饱和及单不饱和脂肪酸含量较高。雌鱼胸肌、鳍肌、骨骼中脂肪酸组成与雄鱼相似,雌鱼皮肤中DHA和EPA含量明显高于雄鱼。结论斑纹光躄鱼的胸肌组织较易吸收多不饱和脂肪酸,骨组织较易吸收饱和及单不饱和脂肪酸;雌性斑纹光躄鱼皮肤中积蓄EPA和DHA高于雄性。

Objective To analyze the fatty acid composition of pectoral muscle,fin muscle,stomach,skin and skeleton in Histrio histrio.Methods The fatty acids of five tissues were methylated with 5%sulfuric acid/methanol solution,and then determined by gas chromatography.Results The contents of saturated,monounsaturated and polyunsaturated fatty acids in five tissues were 42.03%-47.68%,13.63%-20.37%and 29.53%-41.29%,respectively.Polyunsaturated fatty acids were the most abundant in pectoral muscle,while saturated and monounsaturated fatty acids were higher in skeleton.The fatty acid composition of pectoral and fin muscle and skeleton was comparable between female and male fish,but cutaneous content of DHA and EPA was higher in females than in males.Conclusion The pectoral muscle and skeleton of Histrio histrio could absorb more polyunsaturated fatty acids and saturated or monounsaturated fatty acids,respectively.Cutaneous accumulation ofEPA and DHA in females is superior to that in males.