摘要
目的探讨ABO血型对人体血浆中凝血因子Ⅷ(FⅧ)活性与纤维蛋白原(Fib)含量的影响,为新鲜血浆制剂的质量控制以及相关质量标准的建立提供理论指导。方法测定的样品包括单采新鲜血浆与手工分离新鲜血浆,FⅧ活性(FⅧ∶C)与Fib含量均采用凝固法进行测定;扩增标本的ABO基因第6外显子并测序来确定基因型。结果单采新鲜血浆中的FⅧ∶C为(147.421±45.773)%,手工分离新鲜血浆中的FⅧ∶C为(119.083±35.130)%(P<0.05);而单采新鲜血浆中的Fib含量为(2.252±0.381)g/L,手工分离新鲜血浆中的Fib含量为(2.324±0.470)g/L(P>0.05);非O型(A、B、AB型)的单采新鲜血浆与手工分离新鲜血浆中FⅧ∶C分别为(167.048±40.862)%与(129.251±33.503)%,O型单采新鲜血浆与手工分离新鲜血浆中的FⅧ∶C分别为(121.386±38.632)%与(91.589±22.328)%,非O型血浆中的FⅧ∶C均要显著高于O型血浆中的FⅧ∶C(P<0.05);非O型单采新鲜血浆与手工分离新鲜血浆中的Fib含量分别为(2.242±0.385)g/L与(2.329±0.472)g/L,O型单采新鲜血浆与手工分离新鲜血浆中的Fib含量分别为(2.287±0.370)g/L与(2.307±0.462)g/L,非O型血浆的Fib含量与O型血浆的Fib含量均无明显差异(P>0.05)。结论 Fib含量与ABO血型的相关性不大,但FⅧ∶C与ABO血型显著相关,在与FⅧ相关血液制剂的制备及质量控制工作中,应当考虑ABO血型对FⅧ∶C的影响,建议根据ABO血型建立血浆制剂有关FⅧ的质量标准。
Objective Tostudy the effect of ABO blood group on the FⅧ∶C and Fib content in human plasma, so as to provide the oretical guidance for the quality control of fresh plasma products and the establishment of relevant quality standards. Methods Samples determined included fresh plasma collected and fresh plasma separated manually. The FⅧ∶C and Fib content were determined by coagulation method. The exon6 of ABO gene was amplified and sequenced to determine the genotype. Results The FⅧ∶C in fresh plasma collected was(147.421±45.773)%, and that in fresh plasma separated manually was(119.083±35.130)%, showing significant differences(P<0.05). The Fib content in fresh plasma collected was(2.252±0.381)g/L, and that in fresh plasma separated manually was(2.324±0.470)g/L, with no significant differences observed(P>0.05). The FⅧ∶C in non-O type(A, B, AB type) fresh plasma collected and fresh plasma separated manually were(167.048±40.862)% and(129.251±33.503)%, respectively,significantly higher than that in O type fresh plasma collected and fresh plasma separated manually as(121.386±38.632)% and(91.589±22.328)%, respectively. The Fib contents in non-O type fresh plasma collected and fresh plasma separated manually were(2.242±0.385)g/L and(2.329±0.472)g/L, respectively. The Fib contents in O type fresh plasma collected and fresh plasma separated manually were(2.287±0.370)g/L and(2.307±0.462)g/L, respectively,and no significant difference was noticed(P>0.05). Conclusion There was no significant correlation between Fib content and ABO blood group, while FⅧ∶C was significantly correlated with ABO blood group. In the preparation and quality control of FⅧ related blood products, the effect of ABO blood group on the FⅧ∶C should be considered, and the quality standard of FⅧ in plasma products should be established based on the ABO blood group.
作者
兰静
左琴琴
张嫄
李凤琴
张雅莉
徐华
LAN Jing;ZUO Qinqin;ZHANG Yuan;LI Fengqin;ZHANG Yali;XU Hua(Shaanxi Blood Center,Xian 710000,China)
出处
《中国输血杂志》
CAS
2021年第2期117-119,共3页
Chinese Journal of Blood Transfusion