两种方法检测358例贫血患者抗生素类药物性抗体结果分析

Detection of antibiotic antibodies in 358 patients with anemia:comparative analysis of two methods

目的探究某医院住院贫血患者抗生素类药物性抗体的发生频率并分析其检测结果。方法选取某医院临床应用抗生素类药物≥3 d并伴有贫血的住院患者血标本358例,应用药物包被的红细胞法和药物添加法2种方法进行抗生素类药物性抗体的检测,并查阅患者相关临床资料对试验结果进行综合分析。结果在358例患者血标本中,应用药物包被的红细胞法共检测出抗生素类药物性抗体阳性12例,总阳性率为3.35%(12/358)。其中头孢类药物性抗体共检测284例,阳性6例,阳性率为2.11%(6/284),非头孢类药物性抗体共检测74例,阳性6例,阳性率为8.11%(6/74),头孢类药物性抗体与非头孢类药物抗体阳性率比较,差异有统计学意义(P<0.05);12例抗生素类药物性抗体阳性患者,分别按性别、年龄、手术和非手术统计比较,差异均无统计学意义(P>0.05);应用药物添加法所有患者血标本均未检测到抗生素类药物性抗体。结论患者使用抗生素类药物后,部分患者体内会产生相应的抗生素类药物性抗体。因此,加强临床对抗生素类药物性抗体的关注度,对临床抗菌素的应用以及输血治疗的精准性和安全性及有效性具有重要意义。

Objective To investigate the frequency of antibiotic antibodies in hospitalized patients with anemia and analyze the yieldingresults.Methods The blood samples of 358 inpatients with anemia,whohad taken antibiotics for 3 days or more,were selected. The drug-coated red blood cell method and drug addition method were used for the detection of antibodies toantibiotic drug, and the relevant clinical data were consulted for comprehensive analysis of the experimental results.Results Among the 358 blood samples, antibiotic antibodies were detectedin 12 by drug-coated red blood cell method, with ayielding rate of 3.35%(12/358). 6 out of 284 samples subjected to cephalosporin antibodies testing were positive,with a positive rate at 2.11%(6/284), and6 out of 74 samples subjected to non-cephalosporins antibodies testing were positive,with a positive rate at 8.11%(6/74),showingstatistical significance between the above two positive rates(P<0.05).No significant difference was notable among the 12 patients with positive antibioticantibody by gender, age, operation andnon-operation(P>0.05).No antibiotic antibodies were yielded in blood samples bydrug addition method.Conclusion The corresponding antibodiesagainst antibiotic could be produced insome patientsafter takingantibiotics.Therefore, enhancing the clinical attentionto antibiotic antibodies is of great significance to the effective application of clinical antibioticsand the accuracy of blood transfusion therapy.