薯蓣皂苷减轻卵清蛋白诱导的过敏性哮喘小鼠的气道炎症

Dioscin alleviates airway inflammation in ovalbumin-induced allergic asthma mice

目的探讨薯蓣皂苷对卵清蛋白(ovalbumin,OVA)诱导的过敏性哮喘小鼠中过敏性支气管炎的影响及机制。方法24只小鼠随机分为对照组、OVA组、OVA+30 mg/kg薯蓣皂苷组和OVA+60 mg/kg薯蓣皂苷组,每组纳入6只小鼠。全自动生化仪检测各组支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中嗜中性粒细胞、嗜酸性粒细胞和单核细胞的数量;ELISA法检测BALF中促炎因子IL-1β、IL-4、IL-5和TNF-α的含量;PAS染色法观察肺组织黏液分泌情况并对黏液分泌程度进行评分;免疫组化法观察肺组织p-NF-κB p65的表达和分布情况;Western blotting法检测肺组织中p-IκB和NF-κB p65的蛋白表达水平。结果薯蓣皂苷可降低过敏性哮喘小鼠BALF中炎性细胞数量,同时降低BALF中促炎因子IL-1β、IL-4、IL-5和TNF-α的水平以及肺部黏液的分泌和NF-κB的活化水平。结论薯蓣皂苷能减轻过敏性哮喘小鼠的气道炎症,且其抗炎作用与抑制NF-κB活化有关。

Dioscin has various biological activities such as anti-inflammatory, anti-tumor and anti-lipid deposition. This article aimed to explore the effect of dioscin on allergic bronchitis in mice with ovalbumin(OVA)-induced allergic asthmaand its mechanism. Total of 24 mice were divided into control group, OVA group, OVA+30 mg/kg diosgenin group and OVA+60 mg/kg diosgenin group, with 6 mice in each group. Automatic biochemical analyzer was used to detect the number of neutrophils, eosinophils and monocytes in bronchoalveolar lavage fluid(BALF) of each group;ELISA was used to detect the levels of pro-inflammatory factors IL-1β, IL-4, IL-5 and TNF-α in BALF. The secretion of mucus in lung tissues was observed by PAS staining while the expression and distribution of p-NF-κB p65, p-IκB and NF-κB p65 in lung tissues were observed by immunohistochemistry and Western blotting. Data showed that dioscin decreased the number of inflammatory cells in BALF and the levels of proinflammatory cytokines IL-1β, IL-4, IL-5 and TNF-α in BALF, as well as the secretion of mucus and the activation of NF-κB in the lungs of allergic asthmatic mice. In conclusion, dioscin can reduce airway inflammation in mice with allergic asthma, and its anti-inflammatory effect is related to the inhibition of NF-κB activation.