甘薯Sporamin基因家族的结构特征及系统进化分析

Structural Characteristics and Phylogenetic Analysis of Sporamin Gene Family in Sweet Potato

Sporamin基因编码的特异贮存蛋白约占甘薯块根中蛋白总量的80%,是甘薯作物特性的重要氨基酸来源。Sporamin基因与编码KTI(Kunitz-type trypsin inhibitors)型大豆胰蛋白酶抑制剂的基因具有同源性,其蛋白产物具有胰蛋白酶抑制活性和肿瘤抑制活性。本研究鉴定了甘薯基因组中的Sporamin基因,预测并分析其在基因结构上的特征,并将此结构特征与Sporamin基因系统进化关联分析。研究鉴定发现普通甘薯(6X)具有极少的Sporamin基因,这可能是单倍型拼接的冗余或错误导致。全基因组加倍及共线性的关联分析发现,WGT和串联重复是Sporamin基因大量扩增的最主要因素,同时依赖基因丢失进行遗传资源的平衡控制。系统进化分析揭示了不同甘薯Sporamin基因的亲缘关系及进化速率差异,还发现较少比例的WGT重复基因可能导致Sporamin基因进化速率的加快。特异性基因表达分析发现部分持续高表达的Sporamin基因,蛋白互作分析发现部分Sporamin蛋白的可能作用通路。研究为甘薯Sporamin基因在分子生物学、遗传改良等领域的后续研究提供了数据材料,具有重要的参考价值与指导意义。

The specific storage protein encoded by the Sporamin gene accounts for about 80%of the total protein in the sweet potato tuber and is an important source of amino acids for the sweet potato crop characteristics.The Sporamin gene has homology with the gene encoding KTI(Kunitz-type trypsin inhibitors)soybean trypsin inhibitor,and its protein product has trypsin inhibitory activity and tumor suppressor activity.This study identified the Sporamin gene in the sweet potato genome,predicted and analyzed its characteristics in the genetic structure,and correlated its characteristics with the Sporamin gene phylogenetic analysis.The research identified that the common sweet potato(6X)has very few Sporamin genes,which may be caused by the redundancy or error of haplotype splicing.The whole genome duplication and collinear correlation analysis found that WGT and tandem repeat are the most important factors for the large amount of Sporamin gene amplification,while relying on gene loss for balanced control of genetic resources.Phylogenetic analysis revealed the phylogenetic relationship and evolution rate difference of different sweet potato Spoarmin genes,and also found that a small proportion of WGT-derived repetitive genes may lead to the acceleration of Sporamin gene evolution rate.Specific gene expression analysis found some Sporamin genes with continuous high expression,and protein interaction analysis found some possible pathways of Sporamin protein.The research provides data materials for the follow-up research of the sweet potato's Sporamin gene in the fields of molecular biology and genetic improvement,and has important reference value and guiding significance.