嫁接番茄长链非编码RNA(lncRNA)的鉴定

Identification of Long Non-coding RNA(lncRNA)in Grafted Tomato

嫁接是重要的农业栽培措施之一,具有增加产量、提高抗性、改善品质等作用。目前对植物嫁接的分子机理研究已经拓展到RNA水平,但主要集中在mRNA和小RNA上,而对长链非编码RNA(lncRNA)的研究还不多见。本研究对公共数据库中嫁接番茄转录组数据进行分析,分别在接穗(番茄)和砧木(马铃薯)中鉴定出3360和5657个物种特异lncRNA,其中有298和847个lncRNA差异表达,9个lncRNA可在接穗和砧木间移动。进一步分析表明,lncRNA的移动机制与相对表达丰度没有明显关联,lncRNA具有调控嫁接植株代谢过程和花期的潜在作用。本研究结果为嫁接番茄lncRNA的鉴定和功能预测提供理论依据。

Grafting is one of the important methods of agricultural cultivation,which can increase yield,enhance resistance,and improve quality.At present,the research on the molecular mechanism of plant grafting has been extended to the RNA level,and mainly focused on mRNA and small RNA.However,the study of long non-coding RNA is still rare.In this study,the transcriptome data of grafted tomato in public database were analyzed and 3360 and 5657 species-specific lncRNA were identified in scion(tomato)and rootstock(potato),respectively,among which 298 and 874 lncRNA were differentially expressed,and 9 lncRNA could be moved between scion and rootstock.Further analysis showed that the movement mechanism of lncRNA was not significantly correlated with the relative expression abundance,and lncRNA had the potential to regulate the metabolic process and flowering period of grafted plants.The results of this study provided a theoretical basis for the identification and function prediction of lncRNAs in grafted tomato.