伊曲康唑对前列腺癌细胞增殖与迁移的影响

Effects of itraconazole on proliferation and migration of prostate cancer cells

目的探讨伊曲康唑对前列腺癌细胞PC-3增殖与迁移的影响。方法体外培养PC-3细胞,取对数生长期的PC-3细胞,分为实验组、溶剂对照组、空白对照组,实验组和溶剂对照组加入180μL完全培养基,实验组制成20μL不同浓度的伊曲康唑工作液,伊曲康唑浓度分别为2.5、5、10、20、40μmol/L。溶剂对照组制成20μL 2.5%的DMSO稀释液。空白对照组加入200μL完全培养基。37℃、5%CO2细胞培养箱中培养细胞48 h,弃上清。MTT实验测定不同浓度的伊曲康唑对PC-3细胞增殖能力的影响。划痕愈合实验测定伊曲康唑对PC-3细胞迁移能力的影响。结果48 h后,溶剂对照组细胞增殖抑制率为8.54%,实验组浓度为2.5、5、10、20、40μmol/L的伊曲康唑对细胞增殖的抑制率分别为12.22%、23.43%、35.30%、48.05%、60.09%,两组比较有统计学差异(P均<0.05)。24 h后实验组与溶剂对照组细胞的相对迁移距离分别为0.6947±0.0227、1±0.0165,两组比较差异有统计学意义(P<0.05)。结论伊曲康唑可抑制前列腺癌细胞的增殖与迁移。

Objective To investigate the effects and mechanism of itraconazole on the proliferation and migration of prostate cancer PC-3 cells.Methods PC-3 cells were cultured in vitro and divided into the experimental group,solvent control group,and blank control group.Complete medium(180μL)was added to the experimental group and solvent control group.Then,in the experimental group,20μL itraconazole solution with different concentrations(2.5,5,10,20 and 40μmol/L)were made;in the solvent control group,20μL DMSO diluent with the final concentration of 2.5%was made.The cells in the blank control group were added with 200μL complete medium,and were cultured at 37℃in 5%CO2 incubator for 48 h,then,the supernatant was discarded.The effects of different concentrations of itraconazole on the proliferation of PC-3 cells were determined by MTT assay.The effect of itraconazole on the migration ability of PC-3 cells was detected by Scratch healing experiment.Results After 48 h,the proliferation inhibition rate was 8.54%in the solvent control group,and the proliferation inhibition rates of PC-3 cells in the experimental group cultured with 2.5,5,10,20 and 40μmol/L itraconazole were 12.22%,23.43%,35.30%,48.05%,and 60.09%,respectively,which were statistically different from those in the control group(all P<0.05).After 24 h,the relative migration distance of cells in the experimental group and the solvent control group was 0.6947±0.0227 and 1±0.0165,respectively,with statistically significant difference between the two groups(P<0.05).Conclusion Itraconazole inhibits the proliferation and migration of prostate cancer cells.