NPRL2与膀胱癌患者临床病理特征的相关性及对膀胱癌细胞凋亡的影响

Correlation of NPRL2 with cinicopathological features of bladder cancer and its effect on cell apoptosis

目的研究NPRL2与膀胱癌患者临床病理特征的相关性及沉默NPRL2对膀胱癌细胞系Biu87/RT-4凋亡的影响。方法收集本院泌尿外科2014年1月至2019年1月的131例膀胱癌患者癌组织标本,免疫组化检测膀胱癌组织中NPRL2表达情况,分析其与膀胱癌临床病理特征的相关性;检测CP-H068、Biu87和RT-4细胞系中NPRL2的表达,CCK-8法检测沉默NPRL2后细胞的增殖情况,流式细胞术检测凋亡,Western blot检测凋亡相关蛋白表达。结果NPRL2在膀胱癌组织中高表达,为53.44%,与膀胱癌临床分期、分级存在显著相关性(P<0.05);NPRL2在膀胱癌细胞系Biu87/RT-4中的表达显著高于正常膀胱上皮细胞CP-H068(P<0.05);沉默NPRL2后Biu87/RT-4细胞增殖能力下降,凋亡率上升,凋亡相关蛋白JNK、Bax、caspase-9表达量增高,Bcl-2表达受抑制,差异均有统计学意义(P<0.05)。结论膀胱癌中NPRL2表达与膀胱癌临床分期和分级相关,通过沉默膀胱癌细胞Biu87/RT-4中NPRL2的表达,可以有效抑制膀胱癌细胞增殖、促进细胞凋亡。

Objective To investigate the correlation between expression of NPRL2 and clinicopathological features of bladder cancer and the effect of NPRL2 silencing on the apoptosis of bladder cancer cell line Biu87/RT-4.Methods Cancer tissue samples were collected from 131 patients with bladder cancer admitted into our department from January 2014 to January 2019.The expression of NPRL2 in bladder cancer tissues was detected by immunohistochemistry,and the correlation between NPRL2 and the clinicopathological features of bladder cancer was analyzed.The expression of NPRL2 in CP-H068,Biu87 and RT-4 cell lines was detected,and the cell proliferation after NPRL2 silencing was detected by CCK-8 assay.Flow cytometry was used to detect cell apoptosis,and Western blotting to detect the expression of apoptosisrelated proteins.Results NPRL2 was highly expressed in bladder cancer samples,accounting for 53.44%,and the expression was significantly correlated with the clinical stage and grade of bladder cancer(P<0.05).Its expression was also obviously higher in the bladder cancer Biu87 and RT-4 cells than the normal bladder epithelial CP-H068 cells(P<0.05).NPRL2 silencing resulted in decreased proliferation ability,elevated apoptotic rate,and increased protein levels of JNK,Bax and caspase-9,while decreased level of Bcl-2 in the Biu87 and RT-4 cells(P<0.05).Conclusion The expression of NPRL2 is related to the clinical stage and grade of bladder cancer.Silencing NPRL2 in bladder cancer cells Biu87/RT-4 can effectively inhibit the proliferation and promote apoptosis of bladder cancer cells.