1株深海来源Aspergillus versicolor(MCCC 3A00937)化学成分及生物活性研究

Study on the chemical composition and biological activity of a strain of Aspergillus versicolor(MCCC 3A00937)from deep sea

目的研究1株采自西太平洋海山区深海沉积物中真菌Aspergillus versicolor(MCCC3A00937)的次级代谢产物。方法利用薄层色谱法(TLC)、硅胶柱层析法、SephadexLH-20凝胶柱层析法、中压制备液相色谱法(MPLC)、高效液相色谱法(HPLC)等分离方法对该菌株发酵次级代谢产物进行了分离纯化;通过核磁共振(NMR)、高效液相色谱法耦合质谱分析(LCMS)等现代波谱学方法,并结合相关文献进行数据分析,鉴定化合物结构。结果从真菌Aspergillus versicolor(MCCC3A00937)的代谢产物中共分离得到9个化合物,分别是Averantin(1),Averantin-1'-meether(2),2H-6-O-Methylaverufin(3),6-O-Methylaverufin(4),6,8-di-O-methylnidurufin(5),versicolorin B(6),5-methoxysterigmatocystin(7),Brevianamide F(8),Psychrophilin E(9)。活性测试结果,化合物2对HL-60、BEL-704、HCT-116、A549,HeLa和MCF-7细胞系有一定的抑制作用,IC50分别为10.27、4.74、22.12、16.40、8.92、24.26μmol/L;化合物7对MCF-7细胞系有细胞毒性,IC50为12.25μmol/L。

Objective The secondary metabolites of fungus Aspergillus versicolor from the deep sea sediments of the Western Pacific seamounts were studied.Methods The secondary metabolites of the strain were separated and purified by thin layer chromatography(TLC),silica gel column chromatography,SephadexLH-20 gel column chromatography,medium pressure liquid chromatography(MPLC)and high performance liquid chromatography(HPLC).Through modern spectroscopy methods such as NMR and LCMS,combined with relevant literature data analysis the structure of the compound were identified.wThe cytotoxic activity of the compounds was measured by MTT method and SRB method.Results Nine compounds were isolated from the secondary metabolites of Aspergillus versicolor(MCCC3A00937),which were identified as Averantin(1),Averantin-1’-meether(2),2H-6-O-Methylaverufin(3),6-O-Methylaverufin(4),6,8-di-O-methylnidurufin(5),versicolorin B(6),5-methoxysterigmatocystin(7),Brevianamide F(8),Psychrophilin E(9).Activity test results,compound 2 exhibited inhibitory activity against HL-60,BEL-704,HCT-116,A549,HeLa and MCF-7,and the IC50 value were 10.27,4.74,22.12,16.40,8.92,24.26μmol/L,respectively;compound 7 inhibited the growth of MCF-7 cells,and the IC50 value was 12.25μmol/L.